蚕蛹虫草液体培养基的研究

Optimum Medium for Deep Fermentation of Cordyceps Militars

通过对蛹虫草菌丝体进行液体摇瓶培养，以菌丝干重为指标，选择出蚕蛹虫草发酵的最适种子培养基和发酵培养基。最佳斜面种子培养基：马铃薯２０％，葡萄糖２％，蛋白胨１％，ＫＨ２ＰＯ４０．１５％，ＭｇＳＯ４０．０５％，琼脂２％，ＶＢ１０．１％片，ｐＨ自然；或：花生粉１％，蔗糖２％，蛋白胨１％，ＫＨ２ＰＯ４０．０５％，琼脂２％，ｐＨ自然。最佳液体种子培养基：蔗糖２％，麸皮１％，玉米粉１．５％，全脂奶粉０．５％，ＫＨ２ＰＯ４０．０５％，ｐＨ６．５。最佳发酵培养基：玉米粉１２％，白砂糖３％，酵母浸出汁液２％，ＮａＮＯ３０．２％，ＫＣｌ０．１％，ＭｇＳＯ４·７Ｈ２Ｏ０．１％，ｐＨ６．５。

? The deep fermentation of Cordyceps militars(L) Link and the index in culturing the dry mycelia are studied. It is concluded that the optimum inclined medium are potato 20%, glucose 2%, KH2PO4 0.15%, MgSO4 0.05%, agar 2%, VB1 0.01% and natural pH. The optimum medium for growing seeds are maize powder 1.5%, wheat bran 1%, whole milk powder 0.5%, KH2PO4 0.05% and natural pH. The optimum fermented medium are maize powder 12%, sucrose 3%, NaNO3 0.2%, KCl 0.1%, MgSO4·7H2O 0.1%, and pH6.5.