硅藻土助滤剂过滤糖化酶的最佳条件

Optimal Condition of Filtering Glucoamylase with Siliceous Earth

用过滤面积为２２ｃｍ２的过滤装置进行试验。研究中用７２１型分光光度计在６００ｎｍ处测定糖化酶的透光率来衡量滤后糖化酶液澄清程度，运用正交试验确定了硅藻土助滤糖化酶液的最佳预涂量、添加量、粗细比，并对最大过滤量、硅藻土对酶活力的影响进行了研究。试验最佳预涂条件：预涂第１层粗土７０６ｇ／ｍ２，第２层细土１８８ｇ／ｍ２，第３层粗土７０６ｇ／ｍ２；添加量为２０ｇ／ｍ２，粗细比为５∶２。

The filtration of glucoamylase fluid is tested with a filtrating apparatus of 22 cm2 in area. The light penetrability of this fluid is assessed by 721 spectrophotometer with the transparency of the filtered glucoamylase fluid at 600 nm. Optimum condition of precoating and the crudeness /fineness ratio of particles are determined by orthogonal experiment: the precoating of the first and third layer are 706 g/m2 (crude particles) and that of the second layer is 188 g/m2 (fine particles), the quantity of addition is 20 g/L, the crudeness /fineness ratio of particles is 5∶2.