耐高温α-淀粉酶产生菌 B.sp-JF_1 的诱变选育

Mutation of Thermophilic Bacillus spJF1 in Thermostable αamylase Preparation

通过采用紫外线（ＵＶ）、亚硝基胍（ＮＴＧ）和甲基磺酸乙酯（ＥＭＳ）等理化方法对可产生耐高温α－淀粉酶的高温菌Ｂａｃｉｌｕｓｓｐ－ＪＦ１进行诱变选育，得到酶活提高为原菌２．２３倍的一株突变株，命名为Ｂａｃｉｌｕｓｓｐ－ＪＦ１ＵＥＮ－Ｔｒｒ－３。

The thermophilic Bacillus sp-JF1 which produces thermostable α-amylase is cultured through mutation and mutants selection. A mutant named as  Bacillus sp-JF1 UEN-Trr-3 is obtained. The activity of this kind of enzyme is 2.23 times to the original strain through conventional methods, such as UV, EMS, NTG and other physicochemical processes.