填充床式生化反应器连续生产L-丙氨酸

Continuous Production of L-Alanine with Packedbed Reactor

在酶法合成Ｌ丙氨酸过程中，产物Ｌ丙氨酸对酶有抑制作用。本文选用ＰＦＲ型填充床式反应器，以ｋ卡拉胶为包埋材料，包埋ＰｓｅｕｄｏｍｏｎａｓｄａｃｕｎｈａｅＤＱｐ１，在底物为１ｍｏｌ／ＬＬ天冬氨酸，反应条件３７℃、ｐＨ６．０时，连续生产Ｌ丙氨酸。在转化率达到９５％时，计算出固定化细胞和反应器的效率分别为０．０１５ｇＬ丙氨酸／ｇ固定化细胞·ｈ、０．０１３ｇＬ丙氨酸／ｍｌ反应器·ｈ。还建立了填充床反应器停留时间与转化率的数学模型进行系统仿真。并连接ＥｓｃｈｅｒｉｃｈｉａｃｏｌｉＳＦＤ４和ＰｓｅｕｄｏｍｏｎａｓｄａｃｕｎｈａｅＤＱｐ１的固定化细胞柱式反应器，试验了从石油裂解副产品反丁烯二酸连续生产Ｌ丙氨酸。

In the course of producing L-Alanine by enzyme, L-Alanine exerts a strong inhibition to the reaction. In this paper, the continuous L-Alanine production with packed-bed reactor is described. Pseudomonas dacunhae DQ-p1 is immobilized with k-Carrageenan, using the 1mol/L L-Aspartic acid as substrates, under 37 ℃ and pH 6.0. At the convertion rate of 95%, the efficiency of the immobilized cell and the reactor are 0.015g L-Alanine/g·immobilized cell·hr, 0.013g L-Alanine/ml·reactor·hr, respectively. Mathematic modeling and dynamic simulation of the system is implemented. We have also experimented to produce L-Alanine continuously from the fumaric acid (the by-roduct of petroleum decomposition)