蔗渣酶法水解的工艺研究

A Study of the Process for the Emzymatic Hydrolysis of Sugarcane Sugar

甘蔗渣的蒸汽预处理的最适条件是220℃,2,220KPa,水—固比为2:1,每100g蔗渣加1gHSO,处理时间02',蒸汽预处理使大部分半纤维素溶解以及增加酶对纤维素的水解作用。蒸汽预处理后,肥溶解的半纤维素分离,然后对水不溶性底物进行水解,这是一个很有利的工艺选择,由于它减少酶的使用,分离木聚糖和葡萄糖,减少最终产品的抑制物以及降低必要的水解能力。发现最佳水解条件2.5FPU/mL T.reesei C—30纤维素酶,15％W/v水不溶性底物,反应时间48h。这些条件使100g甘蔗渣得到21.1g木糖,31.3g葡萄糖,2.2g纤维二糖和2.8g阿拉伯糖,总糖得率57.4g。

The optimum conditions for the steam pretreament of sugar cane bagasse were 220℃, 2, 220KPa, awater-to-solids ratiio of2, 1g HSO per 100g bagasse and a treatment time of 20 seccods, The steam pretreatment solubilized most of the hemicellulose and increased en-zymic susceptibility of water insoluble residue ( WISR ) was the favoured process option since it reduces enzyme usage, separates xylan and glucan fractions, reduces end product inhibition and reduces the necessary hydrolysis capacity Optimum hydrolysis conditions were found to be 2.5FPU/mL T.reesei C-30 cellulase, 15% w/v WISR and a reaction time of 48 hours, These conditions gaving a total sugar yield of 57.4g.