摘要
目的研究成年豚鼠壶腹处Cajal样细胞(ICLC)的分布及Oddi括约肌(SO)的肌电活动。方法取成年豚鼠壶腹全层铺片,c-Kit免疫细胞化学染色观察ICLC的分布情况;采用黏膜接触电极记录豚鼠SO的肌电活动。结果壶腹的外侧壁和内侧壁均可观察到c-Kit阳性ICLC,位于环形和纵行平滑肌层内以及两层平滑肌之间。肌内的ICLC细胞呈梭形。肌间的ICLC胞体呈椭圆形或三角形,发出3个以上的有分支的突起,这些细胞相互连接排列成网络状,与十二指肠的肌间丛Cajal间质细胞(ICCs)十分相似。在壶腹外侧壁的内面ICLC与十二指肠的深肌丛ICCs相似。壶腹的内侧壁肌层有大量的ICLC,与平滑肌细胞平行走行。在壶腹开口处,ICLC围绕开口形成一个ICLC环。在SO记录到自发节律性的肌电活动波。结论壶腹内的ICLC可能参与SO自主节律性运动的调控并与SO运动障碍的发生有关。
Objective To investigate the distribution of interstitial Cajal-like cells (ICLC) in ampulla and the myoelectrie activities of the sphincter of Oddi (SO) of adult guinea-pigs. Methods C-Kit immunohistochemieal staining was carried out on whole-mount prepara tions of guinea-pigs' ampulla region. The myoelectric activities of SO were recorded by using the contact electrode on mucosa. Result C-Kit positive ICLC were observed in both exterior and inner wall of ampulla. Some of these cells were distributed in the circular and longitudinal smooth muscle cell (SMC) layers assuming a spindle-like shape. Another subgroup of ICLC, which were located between the longi tudinal and circular SMC layers, were shown to have oval or triangle cell bodies with projection of more than three long processes. These cells interlaced into a cellular network, closely resembling the interstitial cells of Cajal (ICEs), around the myenteric nervous plexus (ICC MY) in the duodenum. At the inner side of circular SMC layer of the exterior wall, ICLC were similar to ICCs associated with the deep muscular plexus (ICC-DMP) of small intestine. In the inner wall of the ampulla, a large amount of ICLC were found in the SMC layers or bundles, running parallel with them. At the opening of the ampulla, a number of ICLC surrounded the opening to form a ring. The spontaneous rhythmic myoelectric activities could be recorded in the SO. Conclusion It is suggested that the ICLC might contribute to the regulation of the spontaneous rhythmic contraction, and it might be related to development of motility disorders of the SO.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2008年第9期1069-1073,共5页
Medical Journal of Chinese People's Liberation Army
基金
国家自然科学基金资助项目(30570983)
关键词
Cajal样细胞
奥狄括约肌
肌电活动
interstitial Caial-like cells
sphincter of Oddi
myoelectric activity