超声造影评价肝纤维化的实验研究

The experimental study on the evaluation of liver fibrosis using contrast enhanced sonography

目的探讨超声造影评价大鼠肝纤维化的价值。方法应用四氯化碳诱导大鼠肝纤维化，在反相脉冲谐波模式下，经尾静脉团注超声造影剂，记录造影剂到达肝动脉时间（hepatic artery arrival time，HAAT）、到达门静脉时间（portal vein arrival time，PVAT）、到达肝静脉时间（hepatic vein arrival time，HVAT），并与免疫组化结果进行相关性分析。结果S0～S4期HAAT、PVAT两两比较差异无统计学意义；S3期、S4期HVAT较S0～S1期缩短（P〈0．05）。HVAT与C—IV、LN免疫组化染色阳性反应面积百分比呈负相关，相关系数分别为-0．680，-0．639。结论超声造影对于评价肝纤维化有一定的价值。

Objective To explore the value of evaluating rat liver fibrosis using contrast enhanced sonography. Methods Carbon tetrachloride was used to induce liver fibrosis. Under the mode of pulse inversion harmonic imaging, ultrasound contrast agent was bolus injected through tail vein, hepatic artery arrivai time（ HAAT）, portal vein arrival time（PVAT）, hepatic vein arrival time（HVAT） was recorded, and their relationship with immunohistochemical results were analyzed. Results There was no statistical difference of HAAT, PVAT between two groups among S0, - S4 stage. HVAT was shorter in S3 and S4 stage than in S0 - S2 stage （ P 〈 0.05 ）. HVAT was negative correlated with the percentage of immunohistochemical positive area of C-Ⅳ , LN, the correlation coefficients were - 0. 680 and - 0. 639 respectively. Conclusions Contrast enhanced sonography is useful for the diagnosing liver fibrosis.