壳聚糖液晶膜生物相容性的研究

Study on Biocompatibility of Chitosan Liquid Crystalline Membranes

壳聚糖因具有良好的生物相容性、生物可降解性、无口服毒性等而广泛应用于组织工程领域。近年来一些研究表明,液晶态普遍存在于生命体中。本研究从仿生角度出发,制备出壳聚糖液晶薄膜。采用偏光显微镜和动态接触角测定仪分别研究其织构和亲水性能,并通过体外细胞培养法研究了生物相容性。实验结果表明,壳聚糖液晶薄膜呈现典型的胆甾相液晶织构,与非液晶薄膜相比,它不仅具有更好的亲水性能,而且能更好地促进L929细胞黏附、生长、增殖及分化,表现出良好的生物相容性。本研究为开发新型生物材料和更好地模拟体内微环境提供了新的思路。

Chitosan is a nontoxic,biocompatible and biodegradable polymer,and has been widely used as a functional material in tissue engineering.In recent years,some studies have showed that liquid crystal（LC） substances widely exist in life.In the present paper,lyotropic liquid crystals were employed to prepare biomimetic chitosan LC films.The texture and hydrophilicity of chitosan LC films were studied by polarizing optical microscopy（POM） and dynamic contact angle determinator respectively.In vitro biocompatibility of LC membranes was also studied.The results showed that the LC membranes presented a fingerprint-like texture,their hydrophilicity and the biological response of L929 cells on LC membranes were better than those of non-LC membranes.It is revealed that liquid crystalline biomaterials can provide a microenvironment suitable for cells growth,and are promising for tissue engineering in the future.