气相色谱法测定布格呋喃的含量及有关物质

Determination of Buagafuran and Its Related Substances by Gas Chromatograghy

目的建立气相色谱法测定布格呋喃（AF-5）原料药的含量及有关物质。方法采用HP-5毛细管色谱柱（0.32 mm×30 m,0.25μm）;柱温：程序升温,初始温度120℃,保持1 min,以10℃·min^-1升温至230℃,保持15min;进样器温度：200℃;检测器（FID）温度：250℃;载气：氮气;流速：1.0 mL·min^-1;分流比：20∶1。结果布格呋喃与有关物质的分离良好,方法的线性范围为0.01-1.0 g·L^-1（r=0.999 4）,RSD〈2.0%（n=3）,检测限为0.3ng。结论本法专属性强、简便、准确,易操作,适用于布格呋喃原料药的含量及有关物质的测定。

OBJECTIVE To develop a gas chromatography method for determining buagafuran（AF-5） and its related substances.METHODS The chromatographic analysis was performed using HP-5 capillary column（30 mm×0.32 mm,0.25 μm） with column temperature programmed from 120 ℃（holding for 1 min） to 230 ℃（holding for 15 min）by 10 ℃·min^-1.Injector temperature：200 ℃,Detector（FID）temperature：250 ℃,Carrier gas：nitrogen,Flow rate：1.0 mL·min^-1,Split ratio：20∶1.RESULTS Buagafuran and its related substances was separated.The linear range was 0.01-1.0 g·L6-1（r=0.999 4）,RSD〈2.0%（n=3）,the limit of detection was 1 ng.CONCLUSION The method is selective,simple,accurate and suitable for the determination of buagafuran and its related substances.