HPLC同时测定肿节风中3种成分的含量

Simultaneous HPLC determination of three components in Sarcandra glabra(Thunb.)Nakai

目的:建立 HPLC 法同时测定肿节风中异嗪皮啶-7-O-β-D-葡萄糖苷、异嗪皮啶和山柰酚-3-O-β-D-葡萄糖酸苷的含量。方法:色谱柱:Waters XBridge C_(18)柱(250 mm×4.6 mm,5μm);流动相:0.1%醋酸甲醇溶液(A)-0.1%醋酸水溶液(B),梯度洗脱(0～17 min,A:25%,B:75%;17～65 min,A:25%→55%,B:75%→45%);流速为0.5 mL·min^(-1);检测波长为344 nm;柱温26℃。结果:异嗪皮啶-7-O-β-D-葡萄糖苷、异嗪皮啶和山柰酚-3-O-β-D-葡萄糖酸苷的线性范围分别为0.2～4.0μg(r=0.9999),0.2～4.0μg(r=0.9999),0.5～10μg(r=0.9999);平均回收率(n=5)分别为100.6%(RSD=1.7%),99.8%(RSD=1.1%),99.6%(RSD=0.4%)。结论:所建立的 HPLC 方法操作简便,准确可靠,分离度好,为全面评价肿节风药材的质量提供了实验依据。

Objective：To establish an HPLC method for simultaneous determination of isofraxidin -7 - O -β - D - glucopyranoside, isofraxidin, and kaempferol - 3 - O -β- D - glucuronid in Sarcandra glabra （ Thunb. ） Nakai. Method：HPLC with Waters XBridge C18 column（250 mm ×4.6 mm,5 μm）. The mobile phase was 0. 1% acetic acid in methanol （A） and 0. 1% acetic acid water solution （B） with gradient elution mode at the flow rate of 0. 5 mL · min ^-1. The gradient condition was 0 - 17 min, A ： 25% , B ： 75% ; 17 - 65 min, A ： 25% →55% , B ： 75%→ 45%. The detection wavelength was 344 nm. Column temperature ：26 ℃. Results：The linear ranges of isofraxidin - 7 - O - β - D - glucopyranoside, isofraxidin and kaempferol - 3 - O - β - D - glucuronid were 0. 2 - 4.0 μg （ r = 0. 9999） ,0.2 - 4. 0 μg （ r = 0. 9999 ）, 0.5 - 10 μg （ r = 0. 9999 ）, respectively. The average recoveries were 100.6% （ RSD = 1.7 % ）, 99. 8 % （ RSD = 1.1% ）, 99.6 % （ RSD = 0. 4 % ）, respectively. Conclusion： This method is simple, sensitive and reliable, and it can provide an experimental basis for quality evaluation of Sarcandra glabra （Thunb.） Nakai.