胶束毛细管电泳测定补骨脂药材中补骨脂素和异补骨脂素的含量

MECC determination of psoralen and isopsoralen in Fructus Psoraleae

目的:建立测定补骨脂药材中补骨脂素和异补骨脂素含量的毛细管电泳方法。方法:采用胶束毛细管电泳法,未涂层弹性石英毛细管(75μm×60 cm,有效长度50 cm);压力进样,压力:3.0 kPa;进样时间:5 s;分离电压:22 kV;检测波长:245nm;柱温:25℃;运行缓冲液:含30 mmol·L^(-1)SDS 的50 mmol·L^(-1)硼砂-硼酸溶液(pH 9.0)。结果:测得补骨脂素和异补骨脂素的线性范围分别为5.0～80.0μg·mL^(-1)(r=0.9992)和4.7～75.2μg·mL^(-1)(r=0.9998),平均回收率(n=5)分别为98.7%(RSD=3.3%)和97.5%(RSD=2.9%)。结论:方法准确可靠,适合于补骨脂药材中补骨脂素和异补骨脂素的含量测定。

Objective ：To establish a micellar electrokinetic capillary chromatography （MECC） method for the determination of psoralen and isopsoralen in Fructus Psoraleae. Method：The separation was performed on a fused and uncoated silic capillary of 75 μm ×60 cm （50 cm of effective length）. 50 mmol· L^-1 sodium borate - boracic acid （ pH 9. 0） containing 30 mmol · L^-1 sodium dodecyl sulfate （SDS） was used as running buffer. Samples were injected hydrodynamically （5 s,3.0 kPa） into the capillary. The applied voltage was 22 kV and the wavelength of detection was set at 245 nm. The temperature was kept at 25 ℃. Results：The calibration curves were linear in the range of 5.0 - 80.0 μg·mL^-1 （ r = 0. 9992） for psoralen and 4.7 - 75.2 μg·mL^-1 （ r = 0. 9998 ） for isopsoralen. The average recoveries （n = 5） were 98.7% （RSD = 3.3%） for psoralen and 97.5% （RSD = 2. 9%） for isopsoralen. Conclusion：This method is accurate and reliable, and suitable for the determination of psoralen and isopsoralen in Fructus Psoraleae.