HPLC法测定喜树幼枝中喜树碱含量

HPLC determination of camptothecin in young shoots of Camptotheca acuminata Decne

目的:建立 HPLC 法测定喜树幼枝中喜树碱的含量,并用该方法测定14个种源地喜树幼枝中喜树碱的含量。方法:采用 VP-ODS 色谱柱(150 mm×4.6 mm,5μm)为色谱柱,甲醇-水(62:38)为流动相,流速为1.0 mL·min^(-1),检测波长为254nm,柱温为25℃。结果:喜树碱进样量在0.10～1.00μg范围内线性关系良好(r=0.9999)。结论:本法测定喜树幼枝中喜树碱含量操作简便,结果准确可靠。

Objective：To establish an HPLC method for determining of camptothecin in young shoots of Camptotheca acuminata Decne. Method：VP- ODS column（150 mm ×4. 6 mm,5 μm） was used and the mobile phase consisted of methanol -water（62： 38） at the flow rate of 1.0 mL · min^- 1;The determination wavelength was 254 nm and 25 ℃ for column. Results ： The linear range was 0. 10 - 1.00 μg （ r = 0. 9999 ）. Conclusion ： This method is simple and the result is accurate and reliable for determining of camptothecin.