摘要
研究了pH变化对日本囊对虾(Marsupenaeus japonicus)仔虾离子转运酶(ATPase、Na+-K+-ATPase、V-ATPase和HCO3--ATPase)活力和存活、生长的影响。结果表明:pH(7.1、7.6←8.1→8.6、9.1)变化对日本囊对虾仔虾离子转运酶活力和存活率、体质量增重率的影响显著(F>F0.05),而对照组无明显变化。各处理组仔虾ATPase、Na+-K+-ATPase活力在72h内呈峰值变化,于24h时达到最大值,至72h后趋于稳定,稳定后ATPase活力和对照组差异显著,与pH值呈负相关,而Na+-K+-ATPase活力则恢复至对照组水平;仔虾V-ATPase和HCO3--ATPase活力随着pH变化的增加而变化增大,24h后保持稳定,且与pH值呈负相关。在实验梯度范围内,pH7.6、8.6处理组与对照组仔虾存活率差异不显著,且pH8.6处理组仔虾体质量增重率与对照组也无明显差异。建议日本囊对虾仔虾pH变化范围不应大于0.5。
Effects of pH on iontransporting enzyme activities (total ATPase, Na^+-K^+-ATPase, V- ATPase and HCO3^--ATPase) and survival, growth of Marsupenaeus japonicus postlarvae were investigated. It suggested the osmoregulatory adaptable parameter of postlarvae in pH changing in view of osmotic physiological mechanism and provided the scientific basis of regulation for shrimp aquacultural systems. The results showed that the activities of ATPase, Na^+-K^+-ATPase, V- ATPase, and HCO3^--ATPase were obviously affected by pH variation(7.1,7.6←8. 1→8. 6,9.1), which also had significant effect on its survival rate and weight gain (F〉F0.05). Within 72 h of pH changing, the activities of ATPase, Na^+-K^+-ATPase in each treatment group showed peak change with the sampling time, reached theirs climax at 24 h, and stabilized after 72 h, the ATPase activity showed negative correlation with pH, while the activities of Na^+-K^+ -ATPase came back to the level of the control group; meanwhile the activities of V-ATPase, HCOf-ATPase of postlarvae changed increasingly within 24 h and stabilized afterwards, the activities of V-ATPase and HCO3^-ATPase showed negative relation with pH. In the range of experimental grades, the treatment of pH 7. 6, 8. 6 had no obvious difference with control ones in postlarvae survival rate, and the treatment of pH 8. 6 also showed similar condition with the control group in weight gain. It suggested that the changing scope of pH in Marsupenaeus japonicus postlarvae culturing should be not over 0.5.
出处
《水产学报》
CAS
CSCD
北大核心
2008年第5期758-764,共7页
Journal of Fisheries of China
基金
山东省优秀中青年科学家科研奖励基金项目(2006BS07005)
天津市农业科技成果转化与推广项目(0604020)
关键词
PH
日本囊对虾仔虾
离子转运酶
存活
生长
pH
Marsupenaeus japonicus postlarvae
iontransporting enzyme
survivial rate
growth