大豆苷元对压力负荷性大鼠肾脏保护作用研究

Protective effects of daidzein on abdominal coarctation of the aorta in rats induced by pressure overload

目的:研究大豆苷元(daidzein,DD)对压力负荷性实验大鼠肾脏的保护作用及其机制。方法:采用腹主动脉缩窄法制备大鼠实验模型。ig治疗4WK后处死大鼠,分别检测大鼠血浆及肾脏组织中血管紧张素Ⅱ(AngⅡ)含量、肾脏组织中肾素活性(RA)、一氧化氮(NO)、丙二醛(MDA)含量和超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、钙调神经磷酸酶(CaN)活性。结果:模型组大鼠血浆及肾组织AngⅡ含量明显增高,肾组织RA、MDA含量增高,CaN活性明显增高,肾组织NO含量、SOD、GSH-Px活力显著降低。与模型组比较,DD能显著提高肾组织NO含量,明显抑制血浆及肾组织AngⅡ的产生,降低肾组织RA、MDA含量及CaN的活性,提高肾组织SOD活力、GSH-Px酶活性。结论:DD对腹主动脉缩窄所致实验性大鼠肾脏有保护作用,可能与其清除氧自由基、升高NO含量、降低CaN活性、抑制AngⅡ产生有关。

Objective： To investigate the protective effects and mechanism of daidzein（DD） in rats induced by pressure overload. Methods： abdominal coarctation of the aorta model of rats induced by pressure overload was prepared by constricting abdominal aorta. The operated rats were randomly divided into sham operated control group ,aorta-constricted model group and three DD groups （ 30,60,120 mg/kg）. Four weeks later, all rats were sacrificed and blood was collected by decapitated bleeding, in centrifuge tubes. The serum separated and the kidneys quickly harvested were measured the content of angiotensin Ⅱ respectively ,and the kidneys measured the activity of renin （RA） and the content of nitric oxide（NO） and the content of malondialdehyde （MDA） and superoxide dismutase （SOD） ,the activity of glutathione superoxide （GSH-PX） and calcineurin （CAN）. Results： In model group, the content of Ang Ⅱ were significantly increased in the kidney and serum of rats ; the content of RA and MDA and the activity were markedly increased in kidney. The content of NO and the activity of SOD and GSH- Px were marked inhibited in kidney of model group. Compared with model group, the content of NO were significantly increased in kidney administrated DD group, and the product of Ang Ⅱ were suppressed, the content of RA and MDA and the activity of CaN were depressed.. The activity of SOD and GSH-Px in kidney was inhibited. Conclusion ： DD has protective effects on kidney in rats induced by abdominal coaretation of the aorta and its mechanism may be related to raising NO content and reducing the level of Ang Ⅱ and the activity of CaN and cleansing oxygen free radical.