摘要
目的观察辛基酚对MCF-7乳腺癌细胞周期及周期蛋白表达的影响。方法以MTT试验、流式细胞分析、免疫细胞化学和RT-PCR等方法观察辛基酚对MCF-7乳腺癌细胞增殖、细胞周期相和细胞凋亡、CDK2和CDK4 mRNA表达、Cyclin D1 mRNA及蛋白表达的影响。结果4、8、16μmol/LOP作用MCF-7乳腺癌细胞72h时,细胞增殖率分别为107.31%、168.06%、62.00%,G0/G1期细胞分别为(52.46±6.67)%、(50.19±7.39)%、(67.31±5.47)%,对照组(55.27±7.53)%,细胞凋亡率分别为(4.84±1.12)%、(6.48±1.36)%、(19.26±3.57)%,对照组(5.56±1.125)%,16μmol/LOP减少了细胞中CDK2、CDK4、Cyclin D1 mRNA及Cyclin D1的表达。结论4、8μmol/LOP促进MCF-7乳腺癌细胞增殖,16μmol/LOP则抑制细胞增殖,促进细胞凋亡,这可能与OP影响细胞中CDK2、CDK4、Cyc-lin D1 mRNA及Cyclin D1表达有关。
Objective To observe the effect of octylphenol(OP) on cell proliferation, cell cycle and cyclin expression of MCF-7 breast cancer cells. Methods Using MTT test, flow cytometry, irnmunocytochemistry and RT-PCR to observe the changes of cell proliferation, cell cycle, apoptosis, CDK2,CDK4 and Cyclin D1 mRNA expression, Cyclin D1 protein expression of MCF-7 cells. Results When MCF-7 breast cancer cells were treated with 4,8 and 16 μmol/L OP on 72 h, the proliferation ratio of MCF-7 was 107.31% ,168.06% and 62.00% respectively, MCF-7 cell at G0/G1 were (52. 46 ± 6. 67)%, (50. 19 ± 7. 39)% and (67. 31 ± 5.47)% respectively,while control group was (55.27 ± 7. 53)%. The apoptosis ratio of MCF-7 was (4. 84 ± 1.12)%, (6. 48± 1.36) % and (19. 26 ± 3.57v respectively, and the con trol group was (5.56 ± 1. 125)%. The expression of CDK2, CDK4 and Cyclin D1 mRNA significant decreased when MCF-7 breast cancer cells were treated with 16 μmol/L OP, the expression of Cyclin D1 protein decreased too. Conclusion The proliferation of MCF-7 breast cancer cells increases when treated with 4,8μmol/L OP, but it decreases when treated with 16μmol/L OP. These different effects on MCF- 7 cells maybe related to the expressive regulation of CDK2, CDK4 and Cyclin D1.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2008年第9期621-623,共3页
Cancer Research on Prevention and Treatment