Rb基因、Rb2/p130基因在骨肉瘤中的表达与相关性

Expression and Relativity of Rb,Rb2/p130 Gene in Osteosarcoma

目的探讨骨肉瘤(Osteosarcoma,OS)中Rb基因、Rb2/p130基因表达情况及两者间的相关性。方法用免疫组织化学SP法检测Rb、Rb2/p130基因在62例OS、39例骨软骨瘤及51例非肿瘤患者患旁正常骨组织中的蛋白表达情况。结果1.OS组中pRb、pRb2/p130阳性率分别为38.71%、27.42%;2.骨软骨瘤组中两者的阳性率分别为84.62%、76.92%;3.正常骨组织组中两者的阳性率分别为94.12%、82.35%;4.OS组与骨软骨瘤组及正常骨组织组中二个基因表达差异均有统计学意义(P<0.01);5.在骨肉瘤中二个基因表达呈正相关,有统计学意义(r=0.8743,P<0.01)。结论Rb、Rb2/p130基因在骨肉瘤中以低表达方式存在,它们与骨肉瘤的发生、发展及预后关系密切,它们在骨肉瘤中的表达呈正相关,并且有可能通过共同低表达来促使骨肉瘤的发生、发展,而它们共同作用的机制有待进一步研究。

Objective To evaluate the relationship between the expression of Rb and Rb2/p130 gene in osteosarcoma, and examine their possible effects on carcinogenesis and progression of osteosarcomas. Methods Sixty-two cases of osteosarcoma and 39 eases of osteochondroma and 51 cases of normal bone tissues were examined with the method of SP immunohistochemistry. Results The results of the study showed： 1. The expression rate of Rb, Rb2/p130 gene in osteosarcoma was respectively 38.71% and 27. 42%, and in osteochondroma was respectively was 84. 62% and 76. 92%, and in normal bone tissue- was respectively was 94. 12% and 82.35%. 2. Both the expression rate of Rb and Rb2/p130 gene in os teosarcoma was lower than that in osteochondroma and normal bone tissue （P〈0. 01 ）. 3. There were no significant difference between the expression rates of Rb and Rb2/p130 in osteochondroma and normal bone tissue （P〉0.05）. 4. There was significant positively correlations between the expression of Rb and Rb2/p130 gene in osteosarcoma （r = 0. 5265,P〈0. 01）. Conclusion These results demonstrated low expression of Rb and Rb2/p130 gene may involve in the carcinogenesis and the development of osteosarcoma and can reflect the malignant degree and prognosis of osteosarcoma.