IFN-γ基因+874位点单核苷酸多态性与乳腺癌的相关性

Association of Single Nucleotide Polymorphism of Interferon-gamma Gene +874 Site and Breast Cancer

目的探讨IFN-γ基因+874位点单核苷酸多态性与乳腺癌的相关性。方法采用PCR技术对94例乳腺癌患者及96例正常女性IFN-γ基因+874位点A/T单核苷酸多态性(SNP)进行分析,将PCR产物进行克隆、测序。结果乳腺癌患者IFN-γ基因+874位点TT基因型频率为18.1%,显著高于正常人的8.3%,两者之间比较具有显著性差异(χ2=3.95,P=0.047);乳腺癌病例组T等位基因频率显著高于对照组(χ2=4.984,P=0.026)。进一步分析显示,乳腺癌病例组中不同年龄段基因型频率和等位基因频率相比,均无显著性差异。结论IFN-γ基因+874位点TT基因型可能与乳腺癌的发生相关,T等位基因可能为乳腺癌发生的遗传危险因素。

Objective To explore the relationship between the single nucleotide polymorphism of interferon-gamma gene ＋ 874 site and breast cancer. Methods Using PCR technique to analyze the single nucleotide polymorphism （SNP）of Interferon-gamma Gene ＋ 874 site A/T of 96 normal women and 94 female breast cancer patients, the PCR products were cloned and sequenced. Results The frequency of interferon-gamma gene ＋ 874 site TT genotype was significantly higher in patients with breast cancer than that in the healthy individuals （18. 1% vs 8. 3%,P〈0.05） ; T allele frequency was higher in breast cancer group than in normal group. But the TT genotype had no significant correlation with the age in breast cancer patients. Conclusion TT genotype of IFN-γ＋ 874 site might have a relationship with generation of breast cancer.