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Leptin受体在人牙髓干细胞中表达的实验研究 被引量：2

Expression of Leptin-receptor in human dental pulp stem cells
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摘要 目的:研究体外培养人牙髓干细胞表型特征及生物学性状,检测Leptin受体在牙髓干细胞表面的表达。方法:采用组织块法和酶消化法分离培养人牙髓干细胞;免疫组化法、免疫荧光法检测细胞表面分子的表达;体外诱导分化实验检测细胞多向分化能力。免疫组化法检测Leptin受体在人牙髓干细胞表面的表达。结果:分离获得的牙髓干细胞波形丝蛋白和CD146表达阳性,具有向脂肪细胞和成骨细胞分化的潜能;Leptin受体在牙髓干细胞表面阳性表达。结论:分离培养的牙髓干细胞具有干细胞的表型特征及生物学性状,Leptin受体可表达于牙髓干细胞。 Objective： To isolate, culture and identify human dental pulp stem cells（hDPSCs） and investigate the expression of Leptin-receptor in hDPSCs. Methods ： hDPSCs were cultured with tissue-explants and enzyme-digestion techniques. Immunochemistry and immunofluorescence were used to detect the expression of cytokines in hDPSCs. The differentiation potential was evaluated by adipogenic and osteogenic induction assay. The expression of Leptinreceptor in hDPSCs was detected with strept avidin-biotin peroxidase complex （SABC）. Results： Vimentin and CD146 were expressed in hDPSCs, hDPSCs had the potentials to differentiate into adipocytes and odontoblasts. Positive expression of Leptin-receptor in hDPSCs indicated hDPSCs were target cells for Leptin action. Conclusion ： hDPSCs can be cultured by both tissue-explant and enzyme-digest techniques, carrying characters of undifferentiated mesenchyme cells. The expression of CD146 and Leptin-receptor are detected in hDPSCs.
作者 潘爽 凌均棨
机构地区 广州中山大学口腔医学研究所 广州中山大学光华口腔医学院牙体牙髓科
出处 《实用口腔医学杂志》 CAS CSCD 北大核心 2008年第5期669-672,共4页 Journal of Practical Stomatology
关键词 牙髓千细胞 LEPTIN LEPTIN受体 Human dental pulp stem cells （hDPSCs） Leptin Leptin-receptor
分类号 R78 [医药卫生—口腔医学]
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参考文献9

  • 1Gronthos S, Mankani M, Brahim J, et al. Postnatal human dental pulp stem cells ( DPSCs ) in vitro and in vivo [ J ]. Proc Natl Acad Sci USA, 2000, 97(25) :13625 -13630.
  • 2Gronthos S, Brahim J, Li W, et al. Stem cell properties of human dental pulp stem cells [ J ]. J Dent Res, 2002, 81 (8) :531 -535.
  • 3Gronthos S, Franklin DM, Leddy HA, et al. Surface protein characterization of human adipose tissue-derived stromal cells[J]. J Cell Physiol, 2001,189( 1 ) :54 -63.
  • 4Shi S, Gronthos S. Perivascular niche of postnatal mesenchymal stem cells in human bone marrow and dental pulp [J]. J Bone Miner Res, 2003,18(4) :696 -704.
  • 5Hess R, Pino AM, Rios S, et al. High affinity leptin receptors are present in human mesenchymal stem cells (MSCs) derived from control and osteoporotic donors [ J ]. J Cell Biochem, 2005, 94(1) :50-57.
  • 6Thomas T, Gori F, Khosla S, et al. Leptin acts on human marrow stromal cells to enhance differentiation to osteoblasts and to inhibit differentiation to adipocytes [ J ]. Endocrinology, 1999, 140(4):1630-1368.
  • 7Shi S, Robey PG, Gronthos S. Comparison of human dental pulp and bone marrow stromal stem cells by cDNA microarray analysis [ J ]. Bone, 2001,29 ( 6 ) :532 - 539.
  • 8Batouli S, Miura M, Brahim J, et al. Comparison of stem- cell-mediated osteogenesis and dentinogenesis [ J ]. J Dent Res,2003, 82(12) :976 -981.
  • 9逄键梁,吴补领,张亚庆,柯杰,吴纲.转化生长因子β1对人牙本质基质蛋白1基因转录活性的影响[J].实用口腔医学杂志,2007,23(6):871-875. 被引量：3

二级参考文献14

  • 1王亦菁,金岩,史俊南,贺慧霞,杨锐,周泽渊,王彦亮.人牙髓干细胞培养及其细胞表型的分析研究[J].临床口腔医学杂志,2005,21(1):23-25. 被引量：12
  • 2王亦菁,金岩,史俊南,刘源,轩东英.牙髓干细胞向成牙本质样细胞分化过程中基因表达研究[J].牙体牙髓牙周病学杂志,2005,15(2):59-63. 被引量：10
  • 3逄键梁,吴补领,张亚庆,赵红萍.人牙本质基质蛋白1基因启动子的克隆、测序和活性分析[J].牙体牙髓牙周病学杂志,2005,15(8):428-434. 被引量：7
  • 4DSouza RN, Cavender A, Sunavala G,et al. Gene expression patterns of murine dentin matrix protein 1 ( Dmpl ) and dentinsialophosphoprotein (DSPP) suggest distinct developmental functions in vivo. J Bone Miner Res,1997, 12(12) : 2040
  • 5Unterbrink A, O'Sullivan M, Chen S, et al. TGF beta-1 downregulates DMP-1 and DSPP in odontoblasts. Connect Tissue Res, 2002, 43 (2-3) :354
  • 6Kaartinen MT, Sun W, Kaipatur N, et al. Transglutaminase crosslinking of SIBLING proteins in teeth. J Dent Res, 2005, 84(7) :607
  • 7He G, George A. Dentin matrix protein 1 immobilized on type Ⅰ collagen fibrils facilitates apatite deposition in vitro. J Biol Chem, 2004, 279(12):11649
  • 8He G, Dahl T, Veis A, et al. Dentin matrix protein 1 initiates hydroxyapatite formation in vitro. Connect Tissue Res, 2003, 44 (Suppl 1):240
  • 9Piattelli A, Rubini C, Fioroni M, et al. Transforming growth factor-beta 1 (TGF-beta 1 ) expression in normal healthy pulps and in those with irreversible pulpitis. Int Endod J, 2004, 37(2) :114
  • 10Nie X, Tian W, Zhang Y, et al. Induction of transforming growth factor-beta 1 on dentine pulp cells in different culture patterns. Cell Biol Int, 2006, 30(4) :295

共引文献2

同被引文献29

  • 1陈翠珠,曲强.瘦素在骨髓基质干细胞向成骨细胞分化早期的作用研究[J].中国骨质疏松杂志,2004,10(2):158-161. 被引量：7
  • 2李培兵,金宏,刘佃辛,王先远,许志勤,南文考,王永辉,高兰兴.瘦素对体外大鼠成纤维细胞增殖与胶原合成的影响[J].中国修复重建外科杂志,2005,19(1):20-22. 被引量：16
  • 3Hess R,Pino AM, RI os S,et al. High affinity leptin receptors are p resent in human mesenchymal stem cells (MSCs) derived from control and osteoporotic donors[J]J Cell Bio2chem,2005,94 (1):50-57.
  • 4Sagawa H,Itoh S,Wang W,et al. Enhanced bone bonding of the hydroxyapatite/beta-tricalcium phosphate composite by electrical polarization in rabbit long bone[J].Artif Organs,2010,34(6):491-497.
  • 5Ham rick MW, Della-FeraMA,et al.Leptin treatment induces loss of bone marrow adipocytes and increases bone formation in leptin-deficient ob/ob m ice [J].J Bone Miner Res,2005,20 (6): 994-1001.
  • 6Caetano LJ,Carda?o H,Fonseca JE.Osteoblasts and bone formation[J]. Acta Reumatol Port,2007,32(2): 103-110.
  • 7Hayam IG.The potential osteogenic effects of systemic leptin and insulin administration in streptozotocin-induced diabetic female rats [J].Saudi Med J,2007,28(8): 1185-1190.
  • 8Handschin AE,Trentz OA,Hemm IS, et al. Leptin increases extracellular matrix mineralization of hum an osteoblasts from heterotopic ossification and normal bone [J]. Ann Plast Surg,2007,59 (3):329-333.
  • 9Thomas T,Gori F,Khosla S,et al. Leptin acts on human marrow stromal ceils to enhance differentiation to osteoblasts and to inhibit differentiation to adipocytes[J]. Endocrinology, 1999,140:1630-1638.
  • 10Scheller EL,Song J,Dishowitz MI,et al. Leptin functions peripherally to regulate differentiation of mesenchymal progenitor ceils [J].Stem Cells,2010,28:1071-1080.

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实用口腔医学杂志
2008年 第5期

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