摘要
目的:研究体外培养人牙髓干细胞表型特征及生物学性状,检测Leptin受体在牙髓干细胞表面的表达。方法:采用组织块法和酶消化法分离培养人牙髓干细胞;免疫组化法、免疫荧光法检测细胞表面分子的表达;体外诱导分化实验检测细胞多向分化能力。免疫组化法检测Leptin受体在人牙髓干细胞表面的表达。结果:分离获得的牙髓干细胞波形丝蛋白和CD146表达阳性,具有向脂肪细胞和成骨细胞分化的潜能;Leptin受体在牙髓干细胞表面阳性表达。结论:分离培养的牙髓干细胞具有干细胞的表型特征及生物学性状,Leptin受体可表达于牙髓干细胞。
Objective: To isolate, culture and identify human dental pulp stem cells(hDPSCs) and investigate the expression of Leptin-receptor in hDPSCs. Methods : hDPSCs were cultured with tissue-explants and enzyme-digestion techniques. Immunochemistry and immunofluorescence were used to detect the expression of cytokines in hDPSCs. The differentiation potential was evaluated by adipogenic and osteogenic induction assay. The expression of Leptinreceptor in hDPSCs was detected with strept avidin-biotin peroxidase complex (SABC). Results: Vimentin and CD146 were expressed in hDPSCs, hDPSCs had the potentials to differentiate into adipocytes and odontoblasts. Positive expression of Leptin-receptor in hDPSCs indicated hDPSCs were target cells for Leptin action. Conclusion : hDPSCs can be cultured by both tissue-explant and enzyme-digest techniques, carrying characters of undifferentiated mesenchyme cells. The expression of CD146 and Leptin-receptor are detected in hDPSCs.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2008年第5期669-672,共4页
Journal of Practical Stomatology