吴茱萸碱体外抗肿瘤作用

Research on anti-tumor activity of evodiamine in vitro

目的:研究吴茱萸碱(evodiamine,Evo)的体外抑瘤谱,并初步探讨其抗肿瘤的作用机制。方法:MTT法测定Evo对肿瘤细胞的生长抑制情况;采用光镜和电镜观察Evo对人肺腺癌细胞SPC-A1形态的影响;流式细胞术检测Evo对SPC-A1细胞的抑制作用和细胞周期的影响。结果:Evo对SPC-A1等10种肿瘤细胞有选择性的生长抑制作用,5μg/mL的Evo处理后,细胞变圆,体积变小,分泌颗粒增多,细胞折光性下降,大部分细胞脱壁。透射电镜下可见细胞表面微绒毛消失、核染色质浓集、边聚,核碎裂,并可见凋亡小体;流式细胞仪检测Evo作用后,G0/G1期和S期细胞减少,大量细胞停滞在G2/M期,凋亡细胞增多。结论:Evo选择性的抑制肿瘤细胞的生长,引起SPC-A1细胞典型的凋亡形态学改变并使细胞停滞于G2/M期,其机制可能与诱导细胞凋亡和周期阻滞有关。

AIM： To detect the inhibition of evodiamine on ten human tumor lines and study its anti-tumor mechanism. METHODS： The inhibition of cell gTowth of evodiamine was measured by MTF assay. Morphological changes were observed by optical and electron microscopy. Apoptosis and cell cycle distribution of SPC-Al cells were determined by flow cytometry. RESULTS： Evodiamine had the selective inhibition on the growth of ten tumor lines. SPC-Al cells treated with evodiamine showed typical characteristics of apoptosis, the cells became round and smaller, cell detachment, membrane blebbing, cell shrinkage with a condensed cytoplasm, and vesicle formation （abundant vacuoles with muhivesicular bodies） . In the presence of 0.5 ug/mL evodiamine, a net increase in the percentage of cells at G2/M phase was encountered, and this phase was maintained throughout the treatment time. CONCLUSION： Evodiamine has the selective inhibition on the growth of tumor lines and can induce morphological changes, and G2/M arrest of SPC-Al cells. It is probably related to inducing cell apoptosis and cycle arrest of the tumor.