尿激酶型纤溶酶原激活剂在小白菊内酯抑制胃癌细胞转移中的作用

Effects of urokinase plasminogen activator on metastasis inhibition of gastric cancer cells induced by Parthenolide

目的：探讨小白菊内酯（Par）抑制人胃癌SGC7901细胞转移活性及对尿激酶型纤溶酶原激活剂（uPA）的调控作用。方法：MTT法检测SGC7901细胞增殖能力的变化;倒置显微镜下观察细胞形态;transwell小室法检测细胞迁移和侵袭能力;Western blot法检测细胞uPA蛋白表达;RT-PCR法检测uPA的RNA表达。结果：在100-200μmol/L浓度范围内,Par以浓度和时间依赖方式抑制胃癌细胞增殖,倒置显微镜下见到细胞生长明显受到抑制,细胞的迁移能力和侵袭力均显著下降,穿过人工基底膜的细胞数逐渐减少。随着Par作用时间的延长,SGC7901细胞表达uPA水平逐渐下降。结论：Par可以抑制SGC7901的增殖和转移,uPA在Par降低胃癌细胞转移活性中起重要作用。

AIM： To investigate the effect of urokinase plasminogen activator （uPA） on metastasis inhibition of gastric cancer SGC7901cells induced by parthenolide. METHODS： The changes of SGC7901 cells reproductive activity were analyzed by MTT. Light microscope was used to observe the cell morphological changes. The effects of parthenolide on migration and invasion capacity of SGC7901 cells were determined by using matrigel and transwell system. The expression of uPA was detected by Western blot and RT-PCR assays. RESULTS： Parthenolide inhibited the proliferation of SGC7901 cells in a time-and concentration -dependent manner ranging from 100 to 200 umol/L. Light microscopy showed the suppressing effect on growth of SGC7901 cells. After exposed to parthenolide, the migration and invasion capacity of SGC7901 cells were significantly decreased, the number of cells gradually decreased through the basement membrance. The Western blot assay showed that the expression of uPA protein declined gradually after exposed to parthenolide for various period of time. CONCLUSION： Parthenolide can inhibit the growth and metastasis activity of SGC7901 cells, and uPA played an important role in the latter process.