Construction of a chloroplast protein interaction network and functional mining of photosynthetic proteins in Arabidopsis thaliana

叶绿体是光合作用发生的一个典型植物细胞细胞器。在这研究，在 Arabidopsis thaliana 的 1 808 叶绿体核心蛋白质的一个总数被联合以前出版的研究和我们的自己的预言的结果可靠地识别。我们然后首先基于这些核心蛋白质相互作用构造了一个叶绿体蛋白质相互作用网络。网络有 22 包含了 2 的 925 蛋白质相互作用对 214 蛋白质。160 的一个总数以前， uncharacterized 蛋白质在这个网络被注解。光合的建筑群的子单元被模块化，并且在 photosystem 之中的功能的关系我(希腊语的第二十三个字母)， photosystem II ( PSII )， photosystem 的轻收获建筑群我( LHC 我)并且 photosystem 的轻收获建筑群( LHC II )我能在这个网络从预言的蛋白质相互作用被推出。我们进一步由酵母证实了在未知蛋白质 AT1G52220 和光合的子单元 PSI-D2 之间的一个相互作用二混血儿的分析。我们的叶绿体蛋白质相互作用网络应该为光合的蛋白质的功能的采矿和在在 Arabidopsis 的系统生物学水平的叶绿体相关的函数的调查是有用的。

Chloroplast is a typical plant cell organelle where photosynthesis takes place. In this study, a total of 1 808 chloroplast core proteins in Arabidopsis thaliana were reliably identified by combining the results of previously published studies and our own predictions. We then constructed a chloroplast protein interaction network primarily based on these core protein interactions. The network had 22 925 protein interaction pairs which involved 2 214 proteins. A total of 160 previously uncharacterized proteins were annotated in this network. The subunits of the photosynthetic complexes were modularized, and the functional relationships among photosystem Ⅰ （PSI）, photosystem Ⅱ （PSII）, light harvesting complex of photosystem Ⅰ （LHC Ⅰ） and light harvesting complex of photosystem Ⅰ （LHC Ⅱ） could be deduced from the predicted protein interactions in this network. We further confirmed an interaction between an unknown protein AT1G52220 and a photosynthetic subunit PSI-D2 by yeast two-hybrid analysis. Our chloroplast protein interaction network should be useful for functional mining of photosynthetic proteins and investigation of chloroplast-related functions at the systems biology level in Arabidopsis.