脑 脾 胰 肺 肝组织粗提物对人胃癌细胞侵袭转移能力的影响

Effects of Tissue Extracts of Brain,Spleen,Pancreas,Lung and Liver on the Ability of Metastasis and Invasion of Human Gastric Carcinoma

目的:探讨不同组织粗提物对人胃癌细胞株GASL侵袭转移能力的影响及机制。方法:采用Transwell小室法,比较裸鼠脑、脾、胰、肺、肝组织粗提物对人胃癌细胞株GASL侵袭透膜能力的影响;用明胶酶谱法检测不同组织粗提物对GASL分泌基质金属蛋白酶的作用;用MTT法比较GASL对裸鼠脑、脾、胰、肺、肝组织的粘附能力水平。结果:1)肺、肝、胰、脾、脑组织粗提物及照组GASL透膜的细胞数分别是(10.2±3.1)个、(21.3±2.3)个、(25.4±4.1)个、(9.3±1.6)个、(6.0±0.8)个、(7.2±1.1)个,肝、胰组织粗提物作用组GASL侵袭透膜数多于肺、脾、脑粗提物组及无血清对照组,差异具有显著性(P<0.05)。2)明胶酶实验结果表明:经胰组织粗提物、肝组织粗提物作用后,GASL细胞分泌明胶酶能力较强。3)GASL细胞在体外对肝和胰组织粗提物粘附率较高(P<0.05);结论:肝、胰组织粗提物促进人胃癌细胞GASL分泌明胶酶,并与其有较强粘附作用,可提高GASL侵袭转移能力。

Objective： To study the effects and the mechanism of different tissue extracts on the invasive and metastaticability of gastric carcinoma. Methods： Using the Transwell invasion assay, we observed the influence of different tissue extracts on the invasive ability of GASL cells in vitro. Gelatin zymography was used to detect the secretion and activity of Gelatinase in GASL cells incubated with different tissue extracts. The adhesive property of GASL to the different tissue extracts was measured by MTT assay. Results： Transwell invasion assay showed that the numbers of cells incubated with lung, liver, pancreas, spleen and brain extracts were （10.2±3.1）, （21.3±2.3）, （25.4±4.1）, （9.3±1.6）, （6.0±0.8）, and （7.2±1.1）, respectively. In comparison to the control group and other tissue extracts, the tissue extracts of liver and pancreas promoted the invasive ability of GASL cells （P〈0.05）. After incubation with extracts of liver or pancreas, the secretions of MMP-9 and MMP-2 were up-regulated in GASL cells. The GASL cells showed a higher coefficient of adhesion to the liver and pancreas tissue extract than to the other tissue extracts （P〈0.05）. Conclusion： With a higher coefficient of adhesive property, the liver and pancreas tissue extracts can promote the invasive ability of GASL cells through stimulating the secretion of Gelatinase.