白假丝酵母致食源性疾病的病原学检测与鉴定

Detection and evaluation on etiology of candida albicans from food source disease

目的:探究本次食源性疾病发生的病因,提出需解决的问题。方法:运用"染片指引法"对可疑检样直接作快速镜检,确定优势菌系后选择对口培养基,获得菌株作一次性鉴定、动物与模拟实验、血清定量凝集试验。结果:检样呕吐物和水样便中捕获到革兰染色阳性、卵圆形、芽生细胞的优势菌系,分离、鉴定为白假丝酵母。分离株毒素提取液经小白鼠腹腔注射实验,随着剂量的增加,症状逐渐加重直至死亡。患者恢复期血清定量凝集试验结果均高于急性期4倍以上。结论:综合流行病学调查、临床症状和实验室检测结果,证实本次发生的食源性疾病是由白假丝酵母引起。

Objective：To study the ecology of the food source disease and propose the problems to be solved.Methods：The suspected sample was detected to determine the dominant strain,adopting the methods of ＂dyes the piece direction law＂ and cultured in the favored medium.And the strain was obtained to make the disposable evaluation,do the stimulant experiment and quantitative agglutination test.Results：The sample from the vomit and the water was detected as the Gram positive and determined as Candida albicans.After injecting the toxin,extracted from the Candida albicans,into mouse＇s abdominal cavity,the symptom of the mouse aggravates gradually till its die with the dosage increasing.After dissection of the mouse,the intestines section hyperemia accumulates the fluid is obvious.The levels of serum in 20 recovery patients were 4 times or higher than those in acute period.Conclusion：The food source disease is caused by the Candida albicans based on the comprehensive field investigation,the clinical symptom and the laboratory detection.