心理应激对模拟高原环境下大鼠肺损伤影响的实验研究

Effect of psychological stress on lung injury induced by simulated acute high-altitude hypobaric hypoxia in rats

目的:探讨心理应激对模拟高原低压低氧环境下大鼠肺损伤的影响。方法:雄性SD大鼠随机分为对照组(Con组)、低氧组(Hy组)、心理应激(2次/d)组(Ps1组)、心理应激(2次/d×2 d)组(Ps2组)、心理应激(2次/d)+低氧组(Ps1+Hy组)和心理应激(2次/d×2 d)+低氧组(Ps2+Hy组)。通过测定大鼠右肺湿/干比值、全湿肺/体重比值、右肺含水比值、血管"袖套"面积和血管"袖套"面积/血管面积的比值来评定肺损伤的程度。结果:与Con和Hy组比较,Ps2+Hy组的右肺湿/干比值、肺湿重/体重比值、血管"袖套"面积和血管"袖套"面积/血管面积比值均显著增高(P分别<0.01,0.05)。结论:心理应激可加重急性低压低氧环境导致的大鼠肺损伤。

Objective：To examine the effect of psychological stress on lung injury induced by simulated acute high- altitude hypobaric hypoxia in rats. Methods： Male SD rats were randomly divided into six groups： control （Con group）, hypoxia （Hy group）, psychological stress （twice daily × 1 day） （Ps1 group）, psychological stress （ twice daily ×2 day） （Ps2 group）, psychological stress （ twice daily × 1 day） ＋ hypoxia （ Ps1＋ Hy group）, and psychological stress （twice daily ×2 day） ＋ hypoxia （Ps2 ＋ Hy group）. To assess the tung injury, the wet/dry weight ratio of fight lung, the wet lung/body weight ratio, the water ratio of fight lung, the perivascular cuff area of small vessels accompanied with bronchiole and the perivascular cuff area/ vessels area ratio were determined. Results ： Compared with the Con and Hy group, the wet/dry weight ratio of right lung , the wet lung/body weight ratio, the perivascular cuff area and the perivascular cuff area/vessels area ratio were significantly higher in Ps2 ＋ Hy groups （ P 〈 0.01, P 〈 0.05 ）. Conclusion： Psychological stress exacerbates the lung injury induced by simulated acute high -altitude hypobaric hypoxia in rats.