葛根素对KKA^y小鼠肝脏损害的保护作用

Protective effect of puerarin on liver injury in KKA^y mice

目的观察2型糖尿病动物模型KKAy小鼠肝脏的病理变化及葛根素对其肝脏损害的保护作用,探寻糖尿病治疗新思路——保护肝脏的结构和功能。方法9～11周龄雄性KKAy小鼠随机分为模型组和葛根素组,对照组9～11周龄雄性C57BL/J小鼠,每组20只,测量16、20、24、28周龄小鼠体重,24、28周龄空腹血糖。3组动物分别于24、28周处死,测定甘油三酯(TG)、胆固醇(TCHO)、丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)数值。光镜观察3组小鼠肝脏的病理改变。结果24周龄前,葛根素可以有效控制小鼠体重的增加(P<0.05);28周龄葛根素组小鼠的TCHO、血清ALT和AST比同龄模型组有所降低(P<0.05);普通光镜观察,发现28周龄模型组小鼠肝脏结构紊乱,肝细胞胞浆有明显空泡,肝细胞脂肪变。结论早期应用葛根素,可以改善糖尿病小鼠肝脏结构及功能,对糖尿病造成的肝脏损害具有较好的保护作用。

Objective To study the pathologic changes of liver in type II diabetes model KKA^y mice and the protective effect of puerarin on liver injury in KKA^y mice, and to find a new strategy of diabetes- protecting the structure and function of liver. Methods Male KKAy mice, 9 -11weeks, were divided into model group（n =20） and puerarin group（n =20） randomly. Male C57BL/J mice, 9 - llweeks, were control group（n =20）. Body weight was measured at 16, 20, 24, 28 weeks of age and plasma-glucose was measured at 24, 28 weeks. Mice were sacrificed at 24 and 28 weeks of age respectively, and lipids, alanine aminotransferase（ALT） and aspartate aminotransferase （AST） were measured. Hepatic tissue was morphologically observed by light microscope. Results 1. The body weight of mice was controlled by puerarin before 24 weeks of age （P 〈0.05）. 2. Lipids, ALT and AST of KKAy mice in the puerarin group were lower than that in the model group at 28 weeks of age （P 〈 0.05 ）. 3. At 28 weeks of age, the structure of liver was damaged, and fatty degeneration in the hepatocyte was significant. Conclusion Using puerarin in early period can protect the hepatic structure and function in KKA^y mice.