毛细管电泳小型电流检测池和玻碳微电极的制作及应用

Fabrications of the Carbon_Fiber Microelectrode and Small Cell for Amperometric Detection in Capillary Electrophoresis and Its Application

设计制作了小型电流检测池和玻碳微电极，并对微电极的电流＿电压特性进行了研究，讨论了电泳缓冲溶液对分离的影响；建立了４种对位取代苯酚的胶束电动毛细管色谱＿玻碳微电极探针式电流检测分析方法，除对硝基苯酚的检测限（２６０ｍｇ／Ｌ）较高外，其余３种均＜０２５ｍｇ／Ｌ，回收率在９６％～１０６％之间。

The carbon_fiber microelectrode and small cell for amperometric detection in capillary electrophoresis were fabricated, and the cyclic voltammetric property of the carbon_fiber microelectrode was studied. The assay of para substituted phenols by micellar electrokinetic capillary chromatography and insert amperometric detection at carbon_fiber microelectrode was developed. The detection limits were less than 0.25 mg/L except p _nitrophenol(2.60 mg/L). The recovery ranged from 96%～106%.