莲子心乙醇提取液体外抗氧化活性的研究

Study on Antioxidant Activities of Ethanol Extracts from Lotus Germ in vitro

研究了莲子心乙醇提取液对氧自由基的清除作用及其对脂质过氧化反应(LPO)的抑制作用。结果表明:莲子心中总黄酮含量为20.01mg/g。莲子心乙醇提取液对Fenton体系产生的羟自由基和邻苯三酚自氧化产生的超氧阴离子均有清除作用,半数清除量(IC50)分别为0.195g/ml和0.461g/ml;对小鼠肝、肾匀浆自发性过氧化反应有较好的抑制作用,IC50分别为3.996mg/ml和4.527mg/ml。

The scavenging effects of ethanol extracts from lotus germ on oxygen radicals and their inhibitory effect on lipid peroxidation（LPO） were studied. The results showed that the content of total flavonoids in lotus germ determined by ultraviolet spectrophotometry is 20.01 mg/g, and the extracts can both scavenge hydroxyl free radical（.OH） produced by Fenton reaction and superoxide anion free radical（O2-·） produced by oxidation ofpyrogallol, and their inhibitory concentrations of 50% （IC50） are 0.195 g/ml and 0.461 g/ml, respectively. The extracts also have inhibitory effects on lipid pcroxidation in liver and kidney homogenate spontaneously, and their IC50 values are 3.996 mg/ml and 4.527 mg/ml, respectively.