响应面分析法优化超声提取茶多糖工艺的研究

Optimization of Ultrasonic-assisted Extraction Conditions of Tea Polysaccharides by Using Response Surface Methodology

为优化超声提取茶多糖工艺,在单因素试验基础上,选择料液比,提取温度,提取时间为自变量,茶多糖提取率为响应值,利用Box-Benhnken中心组合试验和响应面分析法,研究各自变量交互作用及其对茶多糖提取率的影响,模拟得到二次多项式回归方程的预测模型,并确定超声提取茶多糖工艺最佳条件为:料液比为1:28,提取温度为60℃,提取时间为70min,在此条件下,茶多糖提取率达到10.46%。

In this study, optimum condition of ultrasonic-assistext extraction of tea polysaccharide（TPS） is opttmizexi by response- surface test. On the basis of single-factor test, the mathematical regression model is established about the dependent variable （exlraction yield of tea polysaccharide ） and independent variables （ratio of material to water, extraction temperature and extration time） through Box-Benhnken canter composite design and respome surface methodology. The optimum extraction conditions as follows： material to water ratio 1：28, extraction temperature 60 ℃, time 70 min. Under these conditions, the yield of tea polysaccaride is 10.46%.