响应面法优化林蛙油酶解工艺及产物分子量分布研究

Study on Optimization of Enzymolysis Technology of Forest Frog Oil by Response Surface Method and Molecular Weight Distribution of Its Hydrolysate

采用Box-Behnken中心组合设计和响应面分析,对木瓜蛋白酶酶解林蛙油的主要影响因素即底物浓度、加酶量、酶解温度、时间、pH值进行多项式回归模型建立和最优化,同时对酶解产物进行质谱分析检测。结果表明最佳工艺条件为:底物浓度1%,加酶量1000U/g,温度60℃,时间3.0h,pH6.5;产物经激光解吸电离飞行时间质谱仪分析,水解液中含有四种不同的物质,分子量分别为712.4、826.6、1101.2和1326.1D。

Box-Benhnken＇ s central composite design and response surface method, polynomial regression model on the main influencing factors to enzymolysis of forest frog oil with papain such as substrate concentration, enzyme dosage,enzymolysis temperature, time and pH value was established and they were optimized. Meanwhile, its enzymatic hydrelysates were determined by mass sepectrometry. The optimal enzymatic hydrolysis conditions are determined as： substrate concentration 1%, enzyme dosage 1000 U/g, temperature 60 ℃, time 3.0 h, pH 6.5. According to determination results of laser desorption ionization timeof-fligh mass spectrometer, the hydrolysates of four different componetns, whose molecular weight are 712.4, 826.6, 1101.2 and 1326.1 D respectively.