羊肚菌胞外多糖抗肿瘤作用的研究

Antitumor Activity of Extracellular Polysaccharides from Morchella esculenta

目的:研究羊肚菌胞外多糖(EPM)对S180荷瘤小鼠的抗肿瘤作用与免疫功能的影响。方法:将昆明种小鼠接种S180腹水瘤后随机分组,灌胃给予200、400mg/kg剂量的EPM,以灌胃生理盐水和环磷酰胺为空白对照组和阳性对照组,研究EPM对荷瘤小鼠的抑瘤率、存活期和免疫功能的影响;通过与肿瘤细胞共培养,观察EPM对肿瘤细胞增殖的影响。结果:EPM能够显著提高荷瘤小鼠的脾脏指数、T-淋巴细胞百分率和巨噬细胞吞噬率;2个剂量的EPM对肿瘤S180的抑制率分别为49.04%和64.42%,并且可以显著延长荷瘤小鼠的存活时间;体外实验发现羊肚菌多糖EPM具有直接杀死S180肿瘤细胞的作用,最高抑制率为46.3%,结论:MEP具有显著的抗肿瘤活性和增强免疫功能。

Objective： To study the antitumor effects of extracellular polysaccharides fromMorchella esculenta （EPM） and the immunological function on Sxs0 tumor-beating mice. Method： EPM was intragastrically given to Sls0-bearing mice at the dose of 200 mg/kg and 400 mg/kg. Normal sodium and cyclophospharnide were taken as blank control and positive control respectively. The tumor weight, immunologic organ weights and life span of S180-bearing mice were measured. S180 tumor cells were incubated with various concentrations of EPM for 24 h, and antiproliferative activities against tumor cells were determined by MTT assay. Result： EPM significantly increases the spleen index, percent of T-lymphocyte, macrophage phagocytic index and prolongs survival time of S180-bearing mice.The inhibiting tumor rates at the low and high does are 49.04% and 64.42% respectively. EPM can kill S^80 tumor cells directly in vitro, with the highest inhibiting rate 46.3% at the dose of 50pg/well.Conclusion： EPM has significant antitumor effects and improves immunological function.