摘要
本研究通过比较老龄动脉粥样硬化大鼠的肾、肝、心和脑组织脂肪酸的变化,尤其是n-3多不饱和脂肪酸(PUFA)和n-6PUFA的变化,了解共轭亚油酸对大鼠组织脂肪酸组成的影响。本实验用SD动脉粥样硬化老龄大鼠,分别添加不同剂量的CLA(低剂量0.5g/kgbw·d;中剂量1.25g/kgbw·d和高剂量2.5g/kgbw·d)组和对照组进行实验,研究CLA对动脉硬化大鼠的肾、肝、心和脑组织脂肪酸含量和组成的改变。实验结果表明:CLA可以升高肾脏、肝脏和脑组织中n-3PUFA的含量,降低肝脏和心脏中n-6PUFA的含量和n-6/n-3的比值,CLA可能取代了组织中部分n-6PUFA。CLA在组织中的沉积各不相同,心脏中CLA的含量可达15.77%,而脑中CLA的含量仅为0.22%～0.53%之间。这些变化可能预示CLA可以改善动脉硬化大鼠的组织脂肪酸的含量和组成。
The aim ofthis study was to detennine the changes in fatty acid composition, in particular n-3 and n-6 polyunsaturated fatty acids （PUFA） in rat kidney, liver, heart and brain to assess and compare the effects of conjugated linoleic acids（CLA） supplementation. The fatty acid composition in the various tissues of aged atheroselerotic rats was determined and compared. The aged rats were fed with high lipid. They were randomly divided into three groups： low dose （0.5g/kg bw＇d）, middle dose （1.25 g/kg bw· d） and high dose （2.5 g/kg bw · d） of CLA in diet and control group. The contents of fatty acids in rats tissues were determined. Tissue compositions of total n-3 PUFA increase in brain, liver and kidney of rats fed CLA. The compositions of total n-6 PUFA and n-6/n-3 values decrease in liver and heart tissues of rats fed with CLA. CLA may substitute partial n- 6PUFA in the tissues. Accumulations of CLA are various in the various tissues. The CLA content in heart reaches 15.77 %, but the CLA content in brain is only 0.22%-0.53%.All these indicated that CLA can influence the fatty acid composition and content in tissues on the atherosclerosis rat.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第9期580-584,共5页
Food Science
基金
国家自然科学基金项目(30460117)
教育部“长江学者和创新团队发展计划”项目(IRT0540)
关键词
共轭亚油酸
老龄大鼠
组织
脂肪酸变化
conjugated linoleic acid： aged rats： tissues： fatty acids
