摘要
目的:建立以高效液相色谱法测定人血浆中头孢吡肟浓度的方法。方法:色谱柱为DiamonsilC18,流动相为20mmol.L-1醋酸铵溶液(冰醋酸调节pH值至5.00)-乙腈(90:10),流速为1.0mL.min-1,检测波长为254nm,进样量为20μL,柱温为室温。结果:头孢吡肟血药浓度在0.2~50.0μg.mL-1范围内线性关系良好(r=0.9996),定量下限为0.2μg.mL-1;平均方法回收率为99.16%(RSD=1.29%),平均提取回收率为85.43%(RSD=2.37%),日内、日间RSD均<10%。结论:本方法简便、灵敏、快速、准确,可用于人血浆中头孢吡肟的药动学和生物利用度研究。
OBJECTIVE: To establish an HPLC method for the determination of cefepime in human plasma. METHODS: The separation of sample was carried on a Diamonsil C18 column with the mobile phase consisted of 20 mmol·L^ -1 ammonium acetate (which was adjusted to pH = 5.00 by acetic acid) - acetonitrile (90 : 10) at a flow rate of 1.0 mL · min^- 1. The detection wavelength was set at 254 nm; the sample size was 20μL, and the column temperature was set at room temperature. RESULTS: The linear range of cefepime was 0.2-50.0μg · mL^-1 (r = 0.999 6), with the lower limit of quantitation at 0.2 μg · mL^ -1. The average methodological recovery was 99.16% (RSD = 1.29% ) and the average extraction recovery was 85.43% (RSD = 2.37%) . The inter-day RSD and intraday RSD were all less than 10%. CONCLUSION: The method was simple, sensitive, rapid and accurate and can be used for the pharmacokinetic and bioavailability study of Cefepime.
出处
《中国药房》
CAS
CSCD
北大核心
2008年第29期2262-2264,共3页
China Pharmacy
基金
全军医学科学技术研究"十一五"计划科技攻关课题基金资助项目(06G023)