HPLC-荧光检测法测定美托洛尔的血药浓度

Determination of Serum Concentration of Metoprolol by HPLC-Fluorescence Detection Method

目的:建立以高效液相色谱-荧光检测法测定美托洛尔血药浓度的方法。方法:色谱柱为Symmetry C18,流动相为乙腈-水-三乙胺-磷酸(110:390:2.5:1.6),荧光检测波长为激发波长(Ex)=265nm、发射波长(Em)=298nm,流速为0.8mL.min-1,进样量为20μL。结果:美托洛尔血药浓度在2.0～100.0μg.L-1范围内线性关系良好(r=0.9996);低、中、高3种浓度的平均相对回收率分别为99.73%、98.21%、99.38%,日内RSD分别为2.89%、2.36%、1.32%,日间RSD分别为3.73%、3.03%、2.25%,最低检测限为1.0μg.L-1。结论:本方法精密、准确、特异性强、回收率高、操作简便,适用于临床血药浓度监测及药动学研究。

OBJECTIVE： To establish a HPLC- fluorescence detection method for the determiniation of the serum concentration of metoprolol. METHODS： The determination was performed on Symmetry C18 column, and the mobile phase consisted of acetonitrile- water triethylamine -phosphoric acid （110 ： 390 ： 2.5 ： 1.6） at a flow rate of 0.8 mL ·min^-1 The excitation wavelength was 265 nm and the emission wavelength 298 nm, and the sample size was 20μL. RESULTS： Good linearity was obtained for metoprolol over the range of 2.0-100.0 μg · L^-1 with correlation coefficient r = 0.999 6. At low, medium and high concentrations, the average recoveries of metoprolol were 99.73%, 98.21% and 99.38%, respectively. The intra- day RSD were 2.89%, 2.36% and 1.32%, respectively, and the inter- day RSD were 3.73%, 3.03% and 2.25% respectively. The lowest detectable limit was 1.0μg · L^-1. CONCLUSION： This method is precise, accurate, specific, simple yet with high recovery, and it is applicable for clinical monitoring of blood concentration and pharmacokinetie study.