放射治疗对宫颈癌耐药相关蛋白表达的影响及临床意义

Effect and Clinical Significance of Radiotherapy on the Expression of Multidrug Resistance-associated Protein in Cervical Cancer

目的:探讨放射治疗对中晚期宫颈癌耐药相关蛋白表达的影响及临床意义。方法:应用免疫组化法检测23例中晚期宫颈癌患者放疗前及放疗30Gy时宫颈癌组织中肺耐药蛋白(LRRP)、谷胱甘肽-S-转移酶(GST-л)、胸苷酸合成酶(TS)、拓扑异构酶Ⅱ(TopoⅡ)的表达。结果:放疗前LRRP的阳性表达率为47.8%,GST-л为56.5%,TS为30.4%,TopoⅡ为60.9%;放疗30Gy时LRRP的阳性表达率为69.6%,GST-л为73.9%,TS为73.9%,明显高于放疗前表达(P<0.05);TopoⅡ阳性表达率为13.0%,明显低于放疗前表达(P<0.05)。结论:放射治疗可以引起宫颈癌耐药相关蛋白表达改变,可能诱导或加强多药耐药(MDR)的形成。

OBJECTIVE： To investigate the effect and clinical significance of radiotherapy on the expression of muhidrug resistance- associated protein （MRP） in advanced cervical cancer. METHODS： The immunohistochemistry method was applied to detect the expression of lung resistance protein （LRRP）, glutathione-S-transferases-π （GST-π）, thymidylate synthetase （TS） and type Ⅱ topoisomerase （Topo Ⅱ ） in 23 patients with advanced cervical cancer before radiotherapy and after receiving 30 Gy dose radiation. RESULTS： The positive expression rates of LRRP, GST - π, TS and Topo Ⅱ before radiotherapy were 47.8%, 56.5%, 30.4% and 60.9%, respectively. The positive expression rates of LRRP, GST- π and TS in patients after 30 Gy dose radiation were 69.6%, 73.9% and 73.9%, respectively, which were signficantly higher as compared before radiotherapy（P〈0.05）. The positive expression rate of Topo Ⅱ was 13.0% after radiation, which was significantly lower as compared before radiotherapy（P〈0.05） . CONCLUSION： Radiotherapy might change the expression of MDR in advanced cervical cancer tissues, induce or enhance MDR.