肝细胞肝癌中环氧合酶-2与半胱氨酸蛋白酶-3的表达及意义

Expression of Cox-2 and Caspase-3 in hepatocellular carcinoma and its significance

目的探讨环氧合酶-2(Cox-2)与半胱氨酸蛋白酶-3(Caspase-3)在肝细胞肝癌(HCC)组织中的表达和在HCC发生、发展中的作用及其关系。方法应用组织芯片技术和免疫组化SP法检测112例HCC组织、72例癌旁组织和30例正常肝组织中Cox-2和Caspase-3的表达。结果Cox-2在HCC组织(73.2%)和癌旁组织(65.3%)中的表达阳性率高于正常肝组织(20.0%),差异有统计学意义(P<0.01),与HCC组织分级有关(P<0.05)。Caspase-3在HCC组织中的表达阳性率(35.7%)低于癌旁组织(77.8%)和正常肝组织(80.0%),差异均具有统计学意义(P<0.01)。Caspase-3表达与HCC的组织分级和HBsAg表达显著相关(P<0.01,P<0.05)。在112例HCC组织中Cox-2表达与Caspase-3表达呈负相关(r=-0.391,P<0.001)。结论HCC组织中Cox-2的高表达与Caspase-3的低表达在HCC的发生、发展过程中均可能起重要作用。乙型肝炎感染与Caspase-3的低表达的相关性及其可能机制有待进一步探讨。

Objective To study the clinical significance and effects of the expression of eyelooxygenase-2（Cox-2） and eysteinyl aspartate specific protease-3 （Caspase-3） in the occurrence and development of hepatoeellular carcinoma（HCC）. Methods The expression of Cox-2 and Caspase-3 were detected with tissue mieroarray by immunohistochemieal method in 112 hepatocellular carcinoma tissues,72 adjacent nontumor tissues and 30 normal liver tissues. Results The expressions rate of Cox-2 in hepatocellular carcinoma tissues （73.2%） and adjacent nontumor tissues （65.3%）was significantly higher than that normal liver tissues （20. 0% ）;The expression of Cox-2 was correlated with Edmondson＇s histological grade（ P 〈 0. 05 ）, but was no correlation with patients＇ age and gender, tumor diameter and metastasis and HBsAg expression. The expressions rate of Caspase-3 in hepatoeellular carcinoma tissues （35.7%） was significantly lower than that adjacent hontumor tissues （77.8% , P 〈 0. 01 ）and normal liver tissues （80. 0% ,P 〈 0. 01 ）. The expression of Caspase-3 was correlated with Edmondson＇s histological grade of carcinoma（P 〈 0. 01 ） and HBsAg expression（ P 〈 0. 05 ）, but was no correlation with patients＇age and gender,tumor diameter and metastasis （P 〉 0. 05 ）. The expression of Cox-2 was negative correlated with Caspase-3 expression in HCC samples （ r = - 0. 391 ,P 〈 0. 001 ）. Conclusions Overexpression of Cox-2 and a low Caspase-3 expression were found in HCC, which indicated that Cox-2 may play an important role by suppression of apoptosis during the occurrence and progression.