摘要
目的寻找逆转胃癌细胞多药耐药的有效方法。方法根据多药耐药基因1(MDR1)的基因序列,设计并体外转录合成2条siRNA,用脂质体转染将其导入人胃癌多药耐药BGC-823细胞内。应用MTT法检测转染后癌细胞生长增殖情况及对阿霉素(ADM)的敏感性,流式细胞仪检测细胞膜表面P-糖蛋白(P-gp)表达和细胞内Rh123的潴留情况。结果转染sh-MDR1-1和sh-MDR1-2后,BGC-823细胞对ADM的IC50均降低,敏感性提高;BGC-823细胞MDR1 mRNA和P-gP表达均显著降低,细胞内Rh123稳态积累量均明显增高;上述效果均以转染sh-MDR1-1序列为著。结论sh-MDR1-1特异性siRNA可抑制BGC-823细胞MDR1表达,此为寻找逆转胃癌细胞多药耐药有效方法奠定了基础。
Objective To investigate the inhibitive effects of RNA interference （RNAi） on the expression of MDR1 gene in gastric cancer BGC-823 cells. Methods Two siRNA targeted MDR1 gone were designed and synthesized by transcription in vitro,then transfected into human gastric cancer BGC-823 cells by using liposome transfection reagents. Drug sensitivity was measured by MTT assay, P-gp expression and intracellular Rh123 accumulation were determined by flow eytometry （FCM）. Results sh-MDRI-1 and sh-MDR1-2 could suppress P-gP expression in human gastric cancer BGC-823 cells. Drug sensitivity and intracellular accumulation of Rh123 increased significantly after sh-MDR1-1 was transfected into BGC-823 cells,while the P-gp expression decreased significantly. The sh-MDR1-1 was more effective in the suppression of MDR1. Conclusion sh-MDR1-1 can suppress the expressions of MDR1 and P-gp in human gastric cancer BGC-823 cells.
出处
《山东医药》
CAS
北大核心
2008年第26期12-14,共3页
Shandong Medical Journal
基金
河南省自然科学基金资助项目(0611042300)
