ISSR分子标记技术在金针菇菌株鉴别中的应用

Application of ISSR in identifying Flammulina velutipes strains

利用简单重复序列区间扩增多态性(ISSR)分子标记对来源不同的6种金针菇(Flammulina velutipes)菌株进行了分子鉴定。从20条ISSR引物中筛选出8条可用引物,共获得136条DNA片段,其中多态性条带111个(占总条带数的81.6%)。供试菌株间遗传相似系数从0.5674(农大04-1和全禾04-1)至0.8947(太行05-1和河北03-1)不等。利用UPGMA软件据此进行聚类分析,可将供试金针菇菌株分为3个组群。结果表明,ISSR分子标记可以有效地用于金针菇生产菌株快速准确鉴别,是金针菇指纹图谱分析的有效工具。

In this paper, ISSR molecular fingerprinting markers were employed to identify six strains of Flammulina velutipes by using 8 primers selected from 20 ISSR primers. A total of 136 DNA fragments were amplified, of which, 111 （81.6% of all bands） were polymorphic. The standard genetic identity among the test strains ranged from 0. 5674 （ between Nongda04-1 and Quanhe04-1 ） to 0. 8947 （ between Taihang05-1 and Hebei03-1 ）. The UPGMA cluster analysis based on the standard genetic distances showed that cultivated F. velutipes strains could be divided into three groups. It was indicated that ISSR marker was an effective tool for the strain identification and fingerprint analysis of F. velutipes.