HPLC法测定复方甘草酸苷分散片中甘草酸苷的含量

Determination of the glycyrrhizin content in compound glycyrrhizin dispersible tablets by HPLC

目的建立HPLC法测定复方甘草酸苷分散片中甘草酸苷的含量。方法采用Agilent Extend-C8(5μm,4.6mm×250mm)色谱柱,以2.2%冰醋酸-乙腈(62:38)为流动相,流速为1.0mL.min-1,检测波长为254nm,外标法计算含量。结果甘草酸苷在0.071～0.536mg.mL-1的浓度范围内呈良好的线性关系,回归方程为:y=1.0×107x+107344(r=0.9996),平均回收率为101.5%,系统精密度RSD为0.03%(n=5)。结论本方法简便可靠,快速,重现性好,适用于复方甘草酸苷分散片中甘草酸苷含量的测定。

OBJECTIVE To Establish an HPLC method for the determination of the glycyrrhizin content in compound glycyrrhizin dispersible tablets. METHOD Agilent Extend-Cs （5μm, 4.6mm × 250mm） was used, and the mobile phase consisted of 2.2 % acetic acid-acetonitrile （62：38）, the flow rate was 1.0mL·min^-1, and the detection wavelength was at 254 nm, calculated with the external method. REUSLTS The linear range was obtained between 0. 071～0. 536mg·mL^-1（r = 0. 9996）, and the average recovery was 101.5 %, the system precision（RSD = 0.03 % ）（n = 5） was good. CONCLUSION This method is simple, convenient, rapid and is suitable for determination of the glycyrrhizin content in compound glycyrrhizin dispersible tablets.