摘要
目的采用高效液相色谱法测定盐酸美利曲辛的含量。方法采用VP-ODS C18色谱柱,流动相为乙腈-甲醇-水(20:20:60),检测波长为254nm。结果在建立的色谱条件下,美利曲辛主峰与杂质峰能完全分离,在浓度为55.2～220.8μg.mL-1时,面积与其浓度线性关系良好(r=0.9991);样品的平均回收率为99.86%,RSD=0.13%(n=9);结论该方法准确,简便快捷,重复性好。可用于盐酸美利曲辛的含量及有关物质测定。
OBJECTIVE To develop an HPLC method for the assay of the content of melitracen hydrochloride. METHODS VP-ODS C18 column was used as the stationary phase. The mobile phase contain aeetonitrilemethanol-water （20：20：60）.The UV detection wavelength was 254nm. RESUTLS Under the described chromatographic condition, melitraeen was completely separated from its impurities.The calidration curve was liner（r = 0. 9991 ） in the range of 55.2--220.8μg·mL^- 1 for melitracen hydrochloride, the average recovery was 99.86 % , RSD = 0.13 % （n = 9）. CONCLUSION The method is simple, rapid and accurate. It could be used for the determination of melitracen hydrochloride and its related substances.
出处
《海峡药学》
2008年第9期40-41,共2页
Strait Pharmaceutical Journal
