非小细胞肺癌K-ras基因点突变的研究

Studies on mutation of K-ras gene in non-small cell lung cancer

目的研究肿瘤组织中K-ras基因点突变在非小细胞肺癌(NSCLC)发生中的作用。方法采用聚合酶链反应—单链构象多态性结合银染技术检测31例NSCLC患者肿瘤组织标本中K-ras基因第12、13、61位密码子的突变情况。结果肺癌组织中K-ras突变率与癌旁正常对照组织比较有显著差异(P<0.01);K-ras基因突变与NSCLC患者年龄、性别、吸烟与否、临床分期及肿瘤细胞分化程度无关,但与病理组织学类型相关。结论K-ras基因突变参与了NSCLC的发病过程,可能是NSCLC发生的早期事件。

Objective To investigate the mutation of K-ras gene in patients with non-small cell lung cancer （NSCLC） and the role of the K-ras gene in the genesis of NSCLC. Methods Using PCR-SSCP methods and silver staining technique , the mutations of the 12,13,61 codes of K-ras gene in 31 cases NSCLC samples and their normal tissues around cancer samples were analysed. Results The mutations of K-ras gene in NSCLC samples were significantly higher than that in normal tissues（ P 〈 0.01 ）. The mutation of K-ras was obvious in adenocarcinomas cell carcinomas, but not significantly associated with patients age, sex, smoking history, clinical stage of NSCLC or cell differentiation. Conclusions K-ras gene mutation may play an importmant role in genesis and progression of NSCLC. Mutation of K-ras gene may be an early event of lung cancer.