CO_2气腹对腹内感染炎症反应的影响

Effect of carbon dioxide pneumoperitoneum on inflammatory responsein intra-abdominal infection

目的研究CO2气腹对腹内感染炎症反应的影响。方法将大肠杆菌和脆弱类杆菌分别注入兔腹腔，建立两种腹内感染动物模型，各型再均分为开腹组、CO2气腹组和对照组。用双抗体夹心法检测各组术前，术后1、2、4、7d血中IL-1水平。结果两种感染模型的开腹组术后第1天IL-1水平显著升高，且达高峰，此后逐渐下降，第7天仍高于注射前（P〈0．01）。开腹组术后第1—7天IL-1水平均显著高于CO2气腹组及对照组（P〈0．01）。大肠杆菌感染及脆弱类杆菌感染模型的CO2气腹组分别于术后第1天、第1～4天IL-1水平显著高于对照组（P〈0．01，〈0．05）。结论CO2气腹对腹内感染动物炎症反应轻，免疫功能影响小。

Objective To study the effect of CO2 pneumoperitoneum on inflammatory response in intra-abdominal infection. Methods Rabbits were injected by escheriehia coli and bacteroides fragilis into abdominal cavities respectively,so as to establish two animal models of intra-abdominal infection. Each model was divided into laparotomy group, pneumoperituneum group and control group . Before operation and 1,2,4,7 d after operation, the blood IL-1 levels of each group were measured by double antibody sandwich method. Results In two animal models, The IL-1 level in laparotomy group increased to maximum at 1 day after operation and then decreased gradual/y, but was still higher than pre-operation level at 7 days after operation（P 〈0.01 ）. The IL-1 levels of laparotomy group were signifieandy higher than those in pneumoperitoneum group and control group at 1 to 7 days after operation （P 〈0. 01 ）. The IL-1 levels in pneumoperitoneum group of escheriehia coli and bacteroides fragilis animal models were significantly higher than those in control group at 1 day （ P 〈 0.01 ） and 1 to 4 day（ P 〈 0.05 ） respectively. Conclusions In pneumoperitoneum group of intra-abdominal infection animal model , the inflammatory response was more weakly and the immune function was affected more slightly and restored more quickly than those in laparotomy group.