间充质干细胞分化为脂肪细胞蛋白组学分析

Proteomic analysis of mesenchymal stem cells differentiation into adipocytes

目的利用蛋白组学技术,分析成脂分化过程中蛋白表达改变,探讨脂肪形成机制。方法以体外诱导人体骨髓间充质干细胞(mesenchymal stem cells,MSCs)分化脂肪细胞作为模型,收集诱导0,1和3周细胞,制成蛋白裂解液;采用双向电泳结合质谱分析方法,以0周为对照组,比较诱导1周或3周蛋白质表达改变。结果成脂分化引起44个蛋白表达上调或下调水平超过2倍。这些蛋白功能类型主要属于代谢(39%)、细胞骨架(11%)、氧化还原(6.8%)、蛋白合成与分解(15.9%)、信号传导(6.8%)和分子伴侣(4.5%)等蛋白。结论生物学信息分析表明,被调节的蛋白在脂肪形成过程中起重要作用;MSCs是研究多能前体细胞向包括前脂肪细胞在内的个体细胞系定向分化的有利工具。

Objective To analyze the protein changes of adipocyte differentiation using proteomic technique in order to explore the mechanism of adipogenesis. Methods The model of adipocyte differentiation of mesenchymal stem cells （MSCs） was used for identifying the changes of proteins following the differentiation in vitro. Protein lysates obtained from differentiated cells were compared after adipocyte differentiation for 0, 1 and 3 weeks by 2-dimensional gel electrophoresis and tandem mass spectroscopy. Results 44 proteins were up or down-regulated over 2-fold in the process of adipocyte differentiation. The majority of the modulated proteins belonged to the following functional categories： metabolic （39 % ）, cytoskeleton （ 11% ）, redox （6.8 % ）, protein synthesis and degradation （ 15.9 % ）, signal transduction （6.8 % ） and molecular chaperones （ 4.5 % ）. Conclusion The modulated proteins play important roles in adipogenesis and MSCs are promising tools to investigate the commitment of multipotent precursor cells into individual cell lineages including preadiopeytes.