慢性温和应激对大鼠海马区胶质细胞蛋白影响

Effect of chronic mild stress on expressions of glial fibrillary acidic protein and S100β protein in hippocampus of rats

目的探讨慢性温和应激对抑郁模型大鼠海马星形胶质细胞内胶质纤维酸性蛋白(GFAP)和酸性钙结合蛋白(S100β)表达的影响。方法选用Wistar成年雄性大鼠30只,应用慢性不可预知的温和应激(CUMS)建立抑郁模型,随机分为对照组和抑郁组,刺激时间8周,跳台实验测试大鼠的学习记忆能力,免疫组化法检测海马GFAP和S100β蛋白的表达。结果抑郁组GFAP和S100β免疫反应产物阳性细胞计数值(32.85±5.57,35.64±9.84)均较对照组(27.03±5.47,27.44±9.46)显著增高(P<0.05),细胞形态有所改变;大鼠跳台实验成绩显示,随着应激时间的延长,反应期明显延长,潜伏期缩短,而错误次数从第4周开始明显增加。结论慢性温和应激可影响大鼠的学习记忆能力,诱发抑郁并引起海马GFAP和S100β蛋白表达的增加。

Objective To investigate the expressions of glial fibrillary acidic protein （GFAP） and acidic calcium - binding protein（S100t3） in hippocampus of rat with stimulation of chronic unpredictable mild stress. Methods Thirty adult male Wister rats were selected for the model construction of chronic unpredictable mild stress and divided into two groups randomly. The depression group was treated with eight weeks of random stimulus. During the course, the influence of stress on learning and memory were observed by the rat step down test and the expression of S10013, GFAP protein in hippocampus were detected by immunohistochemistry. Results The GFAP and S100β immunoreactions positive cell number in depression group were significantly increased compared with that of the control group. The reaction time was significantly longer than that of the control , the pre - reaction time was shorten obviously through the rat step down test, and the error frequence was significantly increased after 4 weeks stress. Conclusion Chronic unpredictable mild stress can affect the ability of learning and memory in rats. It can also induce depression and increase the expression of GFAP and S100β.