左旋卡尼汀对糖尿病大鼠血糖及游离脂肪酸影响

Effects of L-Carnitine on FPG and FFA in diabetes mellitus rats

目的通过观察左旋卡尼汀干预后糖尿病大鼠空腹血糖(FPG)和血清游离脂肪酸(FFA)的变化规律,探讨左旋卡尼汀对糖尿病大鼠FPG和FFA的影响。方法采用区组随机分组的方法将36只大鼠分为对照组(NC组)、糖尿病空白组(DM组)和左旋卡尼汀干预组(L-CN组)各12只。将DM组和L-CN组大鼠按照65mg/(kg.bw)剂量腹腔注射链脲佐菌素(STZ)溶液,72 h后测空腹血糖;对L-CN组大鼠腹腔注射左旋卡尼汀,250mg/(kg.bw)其他组腹腔注射无菌水,观察6周测空腹血糖、体重和血清游离脂肪酸。结果链脲佐菌素制备糖尿病模型成功率为91.7%;观察6周后,3组体重变化值、血清游离脂肪酸含量差异均有统计学意义(P<0.001),3组空腹血糖变化值差异无统计学意义(P>0.05)。结论链脲佐菌素制备糖尿病模型成功率高,并且较稳定;L-CN能降低血清游离脂肪酸和体重,但对空腹血糖影响不大。

Objective To observe the effects of L- Carnitine（L- CN） on FPG and FFA in diabetes mellitus （MD） rats. Methods Thirty - six adult Wistar rats were divided into three groups by randomized blocks design. The DM group and L-CN group were given intraperitoneal injection STZ （65 mg/kg· bw）, and the FPG was measured after seventy- two hours. The L - CN group was given intraperitoneal injection L - CN （250 mg/kg·bw） meanwhile, the others were given intraperitoneal injection sterile water as control. During six weeks observation schedule, FPG, BW and FFA were measured regularly. Results The achievement ratio of DM model by intraperitoneal injection STZ was 91.7 %. After six weeks, the FFA between three groups showed statistical difference（ P 〈 0. 001 ）, and the FPG did not show statistical difference（ P 〉 0.05 ）. Conclusion The achievement ratio of DM model by intraperitoneal injection STZ was high and stable. The L - CN could decrease FFA, as well as BW, but could not effect FPG.