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免疫磁珠分选系统在急性髓系白血病干细胞分离中的应用 被引量：5

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摘要 目的:采用免疫磁珠分选系统(MACS)分离人白血病骨髓CD34+、CD38-干细胞群。方法:收集人白血病骨髓,Percoll密度梯度离心分离单个核细胞,CD34、38磁性标记,MACS分离纯化CD34+、CD38-干细胞群,流式细胞仪检测其纯度和分选前后CD34+、CD38-百分比,计算回收率。结果:分选后的CD34+、CD38-细胞纯度达72.6±3.2%,回收率85.8±1.2%。结论:免疫磁珠分选系统是一种有效的白血病干细胞分离提纯方法,所得细胞纯度比较高。
作者 徐瑞荣 王敬毅 崔兴 王琰 胡述博 李丽珍
机构地区 山东中医药大学附属医院血液科 山东大学齐鲁医院免疫室
出处 《浙江中西医结合杂志》 2008年第10期610-612,共3页 Zhejiang Journal of Integrated Traditional Chinese and Western Medicine
基金 国家自然科学基金资助(30672746)
关键词 白血病干细胞CD34^+ CD38^- 免疫磁珠分选系统
分类号 R733.71 [医药卫生—肿瘤]
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共引文献2

同被引文献54

  • 1舒赛男,魏来,方峰,王宇.免疫磁珠分选系统在分离大鼠骨髓干细胞群中的应用[J].标记免疫分析与临床,2006,13(1):35-37. 被引量：8
  • 2王光平,曹星玉,信红亚,李群,齐振华,陈方平.用Midi MACS方法从白血病患者分选CD34^+/CD123^+细胞[J].中国实验血液学杂志,2006,14(5):969-971. 被引量：8
  • 3冀冰心,徐娟,苏力,刘聪艳,董会卿.自体外周血纯化CD34^+细胞移植治疗进展型多发性硬化[J].中国神经免疫学和神经病学杂志,2007,14(2):69-72. 被引量：3
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浙江中西医结合杂志
2008年 第10期

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