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水牛Follistatin cDNA克隆及序列分析 被引量：3

Buffalo Follistatin cDNA cloning and sequence analysis
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摘要 卵泡抑素(Follistatin,FS)可通过旁分泌和自分泌的方式抑制促卵泡素(FSH)的分泌,从而影响动物的繁殖机能。本研究拟通过分析水牛的FS cDNA序列来分析其繁殖性能低的可能原因。试验参照GenBank公布的奶牛的FS cDNA序列设计引物,通过RT-PCR技术扩增目的基因,再将其亚克隆到pMD-18T克隆载体进行测序。测序结果显示本试验成功获得了水牛的FS cDNA序列。同源性及进化树分析提示FS在哺乳动物进化上高度保守。研究发现两处氨基酸的突变,这可能影响FS正常的生物学功能发挥,从而影响水牛的繁殖机能。 Follistatin （FS） affects animal reproductive mechanisms through inhibiting secretion of Follicle-stinmulating hormone （FSH） in paracrine and autocrine. The objective of this study is to elucidate the low reproductive ability in the Bubalus bubalis by analysis FS cDNA sequence. Primers were designed based on the Bos taurus FS cDNA released in GenBank. The purpose segment in RT- PCR technology was amplified and the target gene was sub-cloned to pMD-18T vector before sequencing. The results indicated that this study had successfully obtained FS cDNA from Bubalus bubalis. Homologous and evolutional analysis showed that FS had a high conservation. It was also found that there were two mutants of amino acids in this study, which may impact the normal biological function of FS, and in turn, affects the reproductive abilities in Bubalus bubalis.
作者 邓继贤 莫毅 杨秀荣 蒋和生 郭亚芬 杨学明 蒋钦杨 谢炳坤 李恭贺 覃广胜 韦英明
机构地区 广西大学动物科学技术学院 中国农业大学动物科技学院
出处 《广西农业生物科学》 CAS CSCD 2008年第3期187-191,共5页 Journal of Guangxi Agricultural and Biological Science
基金 广西自然科学基金项目(桂科自0640014)
关键词 水牛 繁殖性能 卵泡抑素 CDNA 克隆 buffalo reproductive ability Follistatin cDNA cloning
分类号 S823 [农业科学—畜牧学]
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参考文献12

  • 1ROBERTSON D M, KLEIN R, MCLACHLAN R I, et al. The isolation of polypeptides with FSH suppressing activity from bovine follicular fluid which are structurally different to inhibin [J]. Biochem Biophys Res Commun, 1987, 149: 744-749.
  • 2SHIMASAKI S, KOGA M, BUSCAGLIA M L, et al. Follistatin gene expression in the ovary and extragonadal tissues[J]. Mol Endocrinol, 1989, 3:651-659.
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二级参考文献25

  • 1ROBERTSON D M, KLEIN R, DEVOS F L, et al. The isolation of polypeptides with FSH suppressing activity from bovine follicular fluid which are structurally different to inhibin [J]. Biochem Biophys Res Commun, 1987,149:744～749.
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  • 6JASWANT S,GREFFP A. Immunohistochemical distribution of follistatin in dominant and subordinate folliceles and corpus luteum of cattle [J]. Biol Reprod,1998,59:561～570.
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二级引证文献16

广西农业生物科学
2008年 第3期

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