益母草提取液对小鼠遗传物质损伤的保护作用及对淋巴细胞功能的增强作用

Protective effect of Herba Leonuri on genetic damage and enhancement on lymphocyte function in mice

目的:观察中草药益母草抗突变作用和对脾T淋巴细胞增殖的影响。方法:小鼠骨髓嗜多染红细胞微核实验(MNT):30只小鼠随机分为6组(每组5只),即阴性对照组(NS)、环磷酰胺(CP)组(30mg.kg-1)及益母草抗诱变组(益母草1.0、2.0、4.0和8.0g.kg-1+CP30mg.kg-1),按改良方法制片检测微核频率;淋巴细胞转化实验:24只小鼠随机分为4组(每组6只),即生理盐水组、CP组(30mg.kg-1)、益母草水煎液组(2.0g.kg-1)及益母草水煎液+CP组(2.0g.kg-1益母草+CP30mg.kg-1),采用MTT法计算刺激指数(SI)。结果:益母草2.0、4.0和8.0g.kg-1剂量组微核率低于CP组(P<0.05);生理盐水组、益母草水煎液组、益母草水煎液+CP组、CP组的SI分别为1.89±0.19、2.17±0.14、1.73±0.13和1.45±0.09;益母草水煎液组的SI显著高于生理盐水组,差异有显著性(P<0.05);益母草水煎液+CP组SI高于CP组,差异有显著性(P<0.05)。结论:益母草具有抗诱变作用,即对遗传物质具有保护作用,并且益母草能提高淋巴细胞功能。

Objective To investigate the anti-mutation of Chinese medicinal herb Herba Leonuri and its effect on T lymphocyte proliferation in spleen. Methods The micronucleus test of mouse bone marrow cell （MNT） ： thirty mice were divided into six groups （n= 5）, negative control （ NS）, cyclophosphamide group （CP 3.0 mg·kg^-1 ） , Herba Leonuri antimutagenesis groups （Herha Leonuri with dosages of 1.0, 2.0, 4. 0, 8.0 g·kg^-1 ＋ CP 30 mg·kg^-1）. The improved method was used to detect the micronuclei frequency. Lymphocyte transformation test： twenty-four mice were divided into four groups （n=6）, saline control, CP control （30 mg·kg^-1）, Herba Leonuri （2.0 g·kg^-1）, HerhaLeonuri-LCP（2.0g·kg^-1 HerbaLeonuri-＋CP30mg·kg^-1） . MTT assay was used to calculate the stimulation index （SI）. Results The micronuclei frequencies in Herba Leonur 2.0, 4. 0, 8.0 g·kg^-1 groups were lower than that in CP group （P〈0.05） ; the SI in saline control, Herba Leonuri, Herha Leonuri ＋CP, CP groups were 1.89±0. 19, 2. 17 ± 0.14, 1.73 ± 0.13 and 1.45±0. 09, respectively, the difference of SI was significant between Herba Leonuri and saline control groups （P〈0.05） ; the difference of SI was significant between Herba Leonuri ＋CP and CP groups （P〈0.05）. Conclusion Herba Leonuri may protect genetic material from injury caused by CP, namely, showing effective antimutagenesis and yet Herba Leonuri could enhance lymphocyte function.