大鼠骨髓间充质干细胞促肝组织损伤修复的作用

Effect of bone marrow mesenchymal stem cells of rats on recovery of hepatic tissue injury

目的:探讨骨髓间充质干细胞(BMSCs)促肝组织缺血-再灌注损伤修复的作用。为肝脏疾病治疗提供新的思路。方法:①取健康Wistar周龄大鼠的骨髓细胞悬液,进行BMSCs体外扩增培养与分化潜能检测。②健康Wistar成年大鼠随机分为实验组(30只)和对照组(10只)。建立肝组织缺血-再灌注损伤模型,将BMSCs肝局部移植到实验组,对照组移植生理盐水,1周后观察两组肝缺血-再灌注损伤后大鼠的存活率以及肝脏修复情况,并通过蓝色荧光标记观察供体BMSCs在受体内的踪迹,探讨BMSCs促宿主损伤肝组织修复的机制。结果:①分离培养的BMSCs增殖旺盛,纯度较高,且均质性和稳定性好;用诱导剂诱导后,BMSCs具有向成骨细胞和脂肪细胞分化的能力。②实验组大鼠成活率达80%,肝细胞逐渐恢复正常,损伤肝组织得到修复,在宿主恢复的肝组织中发现了较多的带有蓝色荧光的细胞存在,而对照组(未移植BMSCs)大鼠腹腔积水、粘连,成活率达30%。2组成活率比较差异有显著性(P<0.05)。结论:BMSCs在体外易分离培养,具有多向分化的潜能;局部移植BMSCs后,可提高大鼠肝组织缺血-再灌注损伤后的恢复。

Objective To study the effect of bone marrow mesenchymal stem cells （BMSCs） promoting the recovery of injured hepatic tissue and provide a new method for the treatment of liver diseases. Methods （1）BMSCs were cultivated and amplified in vitro from bone marrow cell suspension of rats. Then the potential multi-differentiation of BMSCs was detected.（2）Healthy Wistar rats were randomly divided into experimental group （n= 30） and control group （n=10）. The hepatic ischemia-reperfusion injury model was established. BMSCs were transplanted into isehemia-reperfusion livers of rats in experimental group via local transplantation and saline was transplanted into the rats in control group. The survival rate of rats was observed, as well as the recovery of liver. The mechanism of the reparation of hepatic injury was explored through observation of the trace of DAPI-labeling donator＇s BMSCs in aeceptor＇s body. ＇Results （1）BMSCs not only had a high purification, homogeneity and stability, but also had unlimited proliferation. BMSCs could differentiate into osteoblast and adipoeyte by being cultivated in induced media of osteoblast differentiation and adipocyte differentiation. （2）The survival rate of experimental group was 80%. The injured hepatic tissues were repaired. The blue fluorescent cells were found in the recovery of the host liver tissue. The survival rate of control group （non BMSCs implantation） was up to 30%. The survival rates of the two groups had a significant difference （P〈0.05）. Conclusion BMSCs are easily isolated and cultivated in vitro. BMSCs can improve hepatic ischemia-reperfusion injury via local transplantation.