热休克蛋白70在消化性溃疡及急性糜烂出血性胃炎组织中的表达及意义

Expressions of HSP70 in peptic ulcer and acute erosive hemorrhagic gastritis and their significances

目的:研究热休克蛋白70(HSP70)在消化性溃疡及急性糜烂出血性胃炎患者治疗前、治疗后不同阶段及不同时期的表达,探讨其与消化性溃疡和急性糜烂性胃炎的关系。方法:利用纤维电子胃镜检查选取消化性溃疡活动期患者75例(胃溃疡29例,十二指肠溃疡46例)及急性糜烂出血性胃炎13例,正常胃黏膜组26例,前两组患者进行抗溃疡治疗。用RT-PCR方法检测其治疗前后HSP70及内参GAPDH基因表达,用各样品HSP70与内参GAPDH的积分吸光度比值来表示HSP70mRNA的表达量。结果:消化性溃疡患者HSP70mRNA的表达水平明显高于正常者(P<0.01);治疗后消化性溃疡各期(活动期、愈合期、瘢痕期)HSP70mRNA的表达水平高于治疗前(P<0.05或P<0.01);而治疗后消化性溃疡各期(活动期、愈合期、瘢痕期)及不同时期(治疗后第1个月、第2个月)之间差异无显著性(P>0.05)。急性糜烂出血性胃炎患者HSP70mRNA表达水平高于正常对照(P<0.01);急性糜烂出血性胃炎各期(好转组和痊愈组)治疗后第1个月的HSP70mRNA的表达水平高于治疗前(P<0.01或P<0.05);而好转组和痊愈组比较差异无显著性(P>0.05)。结论:HSP70作为一种应激蛋白,参与了消化性溃疡及急性糜烂性胃炎的修复。

Objective To study the relationship between heat shock protein 70 （HSP70） and the pathogenesis of peptic ulcer disease, acute erosive hemorrhagic gastritis. Methods Three groups were selected by conducting endoscopy： 75 patients with peptic ulcer at active stage （29 cases of gastric ulcer, 46 cases of duodenal ulcer）, 13 patients with acute erosive hemorrhagic gastritis, 26 cases of normal gastric mucosa normal, and the patients in the first two groups were given antiulcer therapy. The expressions of HSP70 and GAPDH mRNA before and after treatment were detected by reverse transcription polymerase chain reaction （RT-PCR）. Results The expression level of HSP70 mRNA in ulcer group was significantly higher than that in normal group （P〈0.01）. The expression levels of HSP70 mRNA in ulcer group at three stages （active, healing, and scar stages） after treatment were significantly higher than those before treatment respectively （P〈0.01 or P〈0.05）. Nevertheless, after treatment, there were no significant differences between various stages （active, healing, and scarring stage） of ulcer group and also between various periods （one month, two months） at each stage （P〉0.05）. The expression level of HSP70 mRNA in gastritis group was higher than that in normal group （P〈0.01）. In gastritis group （improved group and healing group）, the expression levels of HSP70 mRNA one month after treatment were higher than those before treatment （P〈0.01 or P〈0.05）. Conclusion HSP70 as a stress protein, participates in repair of peptic ulcer and acute erosive hamorrhagic gastritis.