MMP-3、MMP-9及TIMP-1在急性心肌梗塞中的表达及其意义

Expressions of MMP-3,MMP-9 and TIMP-1 in patients with acute myocardial infarction and their significances

目的:观察急性心肌梗塞(AMI)患者血清中基质金属蛋白酶-3(MMP-3)、基质金属蛋白酶-9(MMP-9)以及基质金属蛋白酶抑制剂-1(TIMP-1)水平的变化,探讨冠状动脉粥样硬化斑块破裂的机制。方法:选择67例AMI患者,发病3h～5d,选择同期住院非冠心病患者40例作为对照组,应用ELISA法测定血清MMP-3、MMP-9、TIMP-1含量以及血清肌钙蛋白Ⅰ(cTnⅠ)、肌酸激酶-MB(CK-MB)水平。结果:与对照组比较,AMI组血清中MMP-3、MMP-9和TIMP-1含量明显增高(P<0.05或P<0.01);AMI组MMP-3/TIMP-1比值和MMP-9/TIMP-1比值均高于对照组(P<0.05)。MMP-3、MMP-9、TIMP-1的含量与cTnI、CK-MB水平无相关性。结论:MMP-3、MMP-9过度表达以及MMP-3/TIMP-1、MMP-9/TIMP-1比例失衡,参与冠状动脉粥样斑块的破裂和心肌梗塞的发生。

Objective To study the levels of matrix metalloproteinase-3 （MMP-3）, matrix metalloproteinase-9 （MMP-9） and inhibitor of metalloproteinases-1 （TIMP-1） in patients with acute myocardial infarction （AMI） and mechanism of plaque rupture of coronary atherosclerosis. Methods Sixty-seven patients with AMI were selected, average invasion time was 3 h-5 d. 40 cases of non-coronary heart disease were included in control group. The expressions of serum MMP-3, MMP 9, TIMP-1 and the levels of serum cTnⅠ, CK-MB were analyzed by enzymelinked immunosorbent assay. Results The serum MMP-3, MMP 9 and TIMP-1 levels in AMI group were higher than those in control group, there was significant difference between two groups （P〈0.05 or P〈0.01）. MMP-3/ TIMP-1 and MMP-9/TIMP-1 in AMI group were higher than those in control group （P〈0.05）. There was no correlation of serum MMP-3, MMP-9 and TIMP-1 with the levels of cTnⅠ and CK MB. Conclusion The overexpressions of MMP-3, MMP-9 and MMP 3/TIMP-1, MMP-9/TIMP-1 disorder may be involved in the procession of plaque rupture of coronary atherosclerosis and AMI.